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Haplotype reference consortium panel: Practical implications of imputations with large reference panels

Iglesias, AI, van der Lee, SJ, Bonnemaijer, PWM, Hohn, R, Nag, A, Gharahkhani, P, Khawaja, AP, Broer, L, Foster, PJ, Hammond, CJ, Hysi, PG, van Leeuwen, EM, MacGregor, S, Mackey, DA, Mazur, J, Nickels, S, Uitterlinden, AG, Klaver, CCW, Amin, N and van Duijn, CM 2017 , 'Haplotype reference consortium panel: Practical implications of imputations with large reference panels' , Human Mutation, vol. 38, no. 8 , pp. 1025-1032 , doi: 10.1002/humu.23247.

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Recently, the Haplotype Reference Consortium (HRC) released a large imputation panel that allows more accurate imputation of genetic variants. In this study, we compared a set of directly assayed common and rare variants from an exome array to imputed genotypes, that is, 1000 genomes project (1000GP) and HRC. We showed that imputation using the HRC panel improved the concordance between assayed and imputed genotypes at common, and especially, low‐frequency variants. Furthermore, we performed a genome‐wide association meta‐analysis of vertical cup‐disc ratio, a highly heritable endophenotype of glaucoma, in four cohorts using 1000GP and HRC imputations. We compared the results of the meta‐analysis using 1000GP to the meta‐analysis results using HRC. Overall, we found that using HRC imputation significantly improved P values (P = 3.07 × 10−61), particularly for suggestive variants. Both meta‐analyses were performed in the same sample size, yet we found eight genome‐wide significant loci in the HRC‐based meta‐analysis versus seven genome‐wide significant loci in the 1000GP‐based meta‐analysis. This study provides supporting evidence of the new avenues for gene discovery and fine mapping that the HRC imputation panel offers.

Item Type: Article
Authors/Creators:Iglesias, AI and van der Lee, SJ and Bonnemaijer, PWM and Hohn, R and Nag, A and Gharahkhani, P and Khawaja, AP and Broer, L and Foster, PJ and Hammond, CJ and Hysi, PG and van Leeuwen, EM and MacGregor, S and Mackey, DA and Mazur, J and Nickels, S and Uitterlinden, AG and Klaver, CCW and Amin, N and van Duijn, CM
Keywords: 1000 Genomes Project reference panel, Haplotype Reference Consortium, association studies, imputation, vertical cup-disc ratio
Journal or Publication Title: Human Mutation
Publisher: Wiley-Liss
ISSN: 1059-7794
DOI / ID Number: 10.1002/humu.23247
Copyright Information:

Copyright 2017 The Authors

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