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Isolation of alginate lyase-producing bacteria and screening for their potential characteristics as abalone probionts

Amin, M, Bolch, CJ ORCID: 0000-0001-9047-2391, Adams, MB ORCID: 0000-0002-5737-5474 and Burke, CM ORCID: 0000-0002-8262-8728 2017 , 'Isolation of alginate lyase-producing bacteria and screening for their potential characteristics as abalone probionts' , Aquaculture Research, vol. 48, no. 11 , pp. 5614-5623 , doi: 10.1111/are.13383.

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Abstract

This study is aimed at the isolation and screening of alginate lyase-producing bacteria from the gastrointestinal tracts of hybrid abalone, Haliotis rubra x H. laevigata, as probiotic candidates. Six bacterial isolates were detected to produce alginate lyase. Of these, the isolate with the highest alginate-lyase activity was identified as Enterobacter ludwigii strain EN-119, displaying 99% similarity of 16S rDNA sequence. Further assays indicated that E. ludwigii showed good viability and stability when it was incorporated into manufactured pellets and stored at 4°C for 7 days. The isolate also had high tolerance of high salinity (35 mg/L), low pH in simulated stomach juice (5) and to simulated intestinal juice containing surfactants such as bile salts and gastric enzymes (pepsin and trypsin). Additionally, a short, preliminary study indicated that supplementation of E. ludwigii via manufactured pellets improved the total weight gain and specific growth rate of hybrid abalone. These results suggest that E. ludwigii is a potential probiont for shortening the culture period of hybrid abalone.

Item Type: Article
Authors/Creators:Amin, M and Bolch, CJ and Adams, MB and Burke, CM
Keywords: hybrid abalone, probiotic candidate, alginate lyase, Enterobacter ludwigii
Journal or Publication Title: Aquaculture Research
Publisher: Blackwell Publishing Ltd
ISSN: 1355-557X
DOI / ID Number: 10.1111/are.13383
Copyright Information:

Copyright 2017 John Wiley & Sons Ltd

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