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Chemotyping of new hop (Humulus lupulus L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry

Yan, D, Wong, YF, Tedone, L ORCID: 0000-0002-5252-8865, Shellie, RA ORCID: 0000-0002-8470-0893, Marriott, PJ, Whittock, SP and Koutoulis, A ORCID: 0000-0001-8846-4456 2018 , 'Chemotyping of new hop (Humulus lupulus L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry' , Journal of Chromatography A, vol. 1536 , pp. 110-121 , doi: 10.1016/j.chroma.2017.08.020.

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Abstract

Comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry (GC × GC-Q-TOFMS) is employed to profile Humulus lupulus L. (hop) essential oils. Comparison of characterised essential oils allows discrimination among chemotypes. Experimental and commercial hop genotypes displayed distinguishable chemotypic patterns among the volatile secondary metabolites making up their essential oils. In total, 210–306 unique compounds were detected (depending on specific genotype), with 99 of these compounds either positively or tentatively identified. Identified volatile secondary metabolites were grouped into esters, monoterpene hydrocarbons, oxygenated onoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes and ketones. Terpenoids were the dominant chemical families across all hop genotypes analysed, representing between 67% and 90% of the total ion count. The multidimensional chromatographic profiles of hop essential oilsare extremely information-rich, making GC × GC-Q-TOFMS useful for fast screening of new hybrid hop genotypes, and therefore informing breeding strategies to derive new commercial hop cultivars for the development of distinctive and desirable beers.

Item Type: Article
Authors/Creators:Yan, D and Wong, YF and Tedone, L and Shellie, RA and Marriott, PJ and Whittock, SP and Koutoulis, A
Keywords: hop, Humulus lupulus L., comprehensive two-dimensional gas chromatography, accurate mass time-of-flight mass spectrometry, hop chemotypes, hop genotypes
Journal or Publication Title: Journal of Chromatography A
Publisher: Elsevier Science Bv
ISSN: 0021-9673
DOI / ID Number: 10.1016/j.chroma.2017.08.020
Copyright Information:

Copyright 2017 Elsevier B.V.

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