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Reduced bone formation markers, and altered trabecular and cortical bone mineral densities of non-paretic femurs observed in rats with ischemic stroke: A randomized controlled pilot study
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ORCID: 0000-0002-2512-7724 2017
, 'Reduced bone formation markers, and altered trabecular and cortical bone mineral densities of non-paretic femurs observed in rats with ischemic stroke: A randomized controlled pilot study'
, PLoS ONE, vol. 12, no. 3
, pp. 1-14
, doi: https://doi.org/10.1371/journal.pone.0172889.
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Abstract
Background:Immobility and neural damage likely contribute to accelerated bone loss after stroke, and subsequent heightened fracture risk in humans.Objective:To investigate the skeletal effect of middle cerebral artery occlusion (MCAo) stroke in rats and examine its utility as a model of human post-stroke bone loss.Methods:Twenty 15-week old spontaneously hypertensive male rats were randomized to MCAo or sham surgery controls. Primary outcome: group differences in trabecular bone volume fraction (BV/TV) measured by Micro-CT (10.5 micron istropic voxel size) at the ultra-distal femur of stroke affected left legs at day 28. Neurological impairments (stroke behavior and foot-faults) and physical activity (cage monitoring) were assessed at baseline, and days 1 and 27. Serum bone turnover markers (formation: N-terminal propeptide of type 1 procollagen, PINP; resorption: C-terminal telopeptide of type 1 collagen, CTX) were assessed at baseline, and days 7 and 27.Results:No effect of stroke was observed on BV/TV or physical activity, but PINP decreased by -24.5% (IQR -34.1, -10.5, p = 0.046) at day 27. In controls, cortical bone volume (5.2%, IQR 3.2, 6.9) and total volume (6.4%, IQR 1.2, 7.6) were higher in right legs compared to left legs, but these side-to-side differences were not evident in stroke animals.Conclusion:MCAo may negatively affect bone formation. Further investigation of limb use and physical activity patterns after MCAo is required to determine the utility of this current model as a representation of human post-stroke bone loss.
| Item Type: | Article |
|---|---|
| Authors/Creators: | Borschmann, KN and Rewell, SS and Iuliano, S and Ghasem-Zadeh, A and Davey, RA and Ho, H and Skeers, PN and Bernhardt, J and Howells, DW |
| Journal or Publication Title: | PLoS ONE |
| Publisher: | Public Library of Science |
| ISSN: | 1932-6203 |
| DOI / ID Number: | https://doi.org/10.1371/journal.pone.0172889 |
| Copyright Information: | © 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ |
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| Item Statistics: | View statistics for this item |
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