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Single cell RNA sequencing of stem cell-derived retinal ganglion cells

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Daniszewski, M, Senabouth, A, Nguyen, QH, Crombie, DE, Lukowski, SW, Kulkarni, T, Sluch, VM, Jabbari, JS, Chamling, X, Zack, DJ, Pebay, A, Powell, JE and Hewitt, AW ORCID: 0000-0002-5123-5999 2018 , 'Single cell RNA sequencing of stem cell-derived retinal ganglion cells' , Scientific Data, vol. 5 , pp. 1-9 , doi: 10.1038/sdata.2018.13.

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Abstract

We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.

Item Type: Article
Authors/Creators:Daniszewski, M and Senabouth, A and Nguyen, QH and Crombie, DE and Lukowski, SW and Kulkarni, T and Sluch, VM and Jabbari, JS and Chamling, X and Zack, DJ and Pebay, A and Powell, JE and Hewitt, AW
Journal or Publication Title: Scientific Data
Publisher: Nature Publishing Group
ISSN: 2052-4463
DOI / ID Number: 10.1038/sdata.2018.13
Copyright Information:

© 2018 The Authors. © 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Metadata Licensed under Creative Commons Attribution CC0 1.0 Universal (CC0 1.0) https://creativecommons.org/publicdomain/zero/1.0/

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