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Assessment of the binding performance of histamine-imprinted microspheres by frontal analysis capillary electrophoresis

Romano, EF, Quirino, JP ORCID: 0000-0002-8275-9672, Holdsworth, JL, So, RC and Holdsworth, CI 2017 , 'Assessment of the binding performance of histamine-imprinted microspheres by frontal analysis capillary electrophoresis' , Electrophoresis, vol. 38, no. 9-10 , pp. 1251-1259 , doi: 10.1002/elps.201600448.

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Abstract

Frontal analysis capillary electrophoresis was used to evaluate the binding performance of molecularly imprinted microspheres (MIM) toward its template histamine and analogs at pH 7, and compared to the high performance liquid chromatographic method. In both methods, batch binding was employed and the binding parameters were calculated from the measured concentration of unbound amine analytes and afforded comparable histamine equilibrium dissociation constants (Kd ∼ 0.4 mM). FACE was easily carried out at shorter binding equilibration time (i.e. 30 min) and without the need to separate the microspheres, circumventing laborious and, in the case of the system under study, inefficient sample filtration. It also allowed for competitive binding studies by virtue of its ability to distinctly separate intact microspheres and all tested amines which could not be resolved in HPLC. Kd’s for nonimprinted (control) microspheres (NIM) from FACE and HPLC were also comparable (∼ 0.6 mM) but at higher histamine concentrations, HPLC gave lower histamine binding. This discrepancy was attributed to inefficient filtration of the batch binding samples prior to HPLC analysis resulting in an over-estimation of the concentration of free histamine brought about by the presence of unfiltered histamine-bound microspheres.

Item Type: Article
Authors/Creators:Romano, EF and Quirino, JP and Holdsworth, JL and So, RC and Holdsworth, CI
Keywords: materials, capillary electrophoresis, frontal analysis capillary electrophoresis, histamine, histamine-imprinted microspheres, molecularly imprinted polymers
Journal or Publication Title: Electrophoresis
Publisher: Wiley-V C H Verlag Gmbh
ISSN: 0173-0835
DOI / ID Number: 10.1002/elps.201600448
Copyright Information:

Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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