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Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography

Creese, ME, Creese, MJ, Foley, JP, Cortes, HJ, Hilder, EF ORCID: 0000-0002-4444-7870, Shellie, RA ORCID: 0000-0002-8470-0893 and Breadmore, MC ORCID: 0000-0001-5591-4326 2017 , 'Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography' , Analytical Chemistry, vol. 89, no. 2 , pp. 1123-1130 , doi: 10.1021/acs.analchem.6b03279.

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Abstract

Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6-14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30-55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.

Item Type: Article
Authors/Creators:Creese, ME and Creese, MJ and Foley, JP and Cortes, HJ and Hilder, EF and Shellie, RA and Breadmore, MC
Keywords: chromatography, heat transfer, liquid chromatography
Journal or Publication Title: Analytical Chemistry
Publisher: Amer Chemical Soc
ISSN: 0003-2700
DOI / ID Number: 10.1021/acs.analchem.6b03279
Copyright Information:

© 2016 American Chemical Society

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