Open Access Repository
A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins
Downloads
Downloads per month over past year
![]() |
PDF
0030_-_0035_Pat...pdf | Request a copy Full text restricted Available under University of Tasmania Standard License. |
Abstract
A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
molecular weight and unfractionated heparins.
© 2007 Elsevier B.V. All rights reserved.
Item Type: | Article |
---|---|
Authors/Creators: | Patel, RP and Narkowicz, CK and Hutchinson, JP and Hilder, EF and Jacobson, GA |
Keywords: | Low molecular weight heparin; Dalteparin; Heparin; Capillary electrophoresis; Batch-to-batch variations |
Journal or Publication Title: | Journal of Pharmaceutical and Biomedical Analysis |
ISSN: | 0731-7085 |
DOI / ID Number: | 10.1016/j.jpba.2007.10.009 |
Additional Information: | The definitive version is available at http://www.sciencedirect.com |
Item Statistics: | View statistics for this item |
Actions (login required)
![]() |
Item Control Page |