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Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma-mass spectrometry

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Abstract
The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin-1. The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n=7), and the detection limit was 14ngmL-1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.
Item Type: | Article |
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Authors/Creators: | Bishop, DP and Blanes, L and Wilson, AB and Wilbanks, T and Killeen, K and Grimm, R and Wenzel, R and Major, D and Macka, M and Clarke, D and Schmid, R and Cole, N and Doble, PA |
Keywords: | NanoLC-ICP–MS, microfluidics, chip HPLC, cobalamin |
Journal or Publication Title: | Journal of Chromatography A |
Publisher: | Elsevier Science Bv |
ISSN: | 0021-9673 |
DOI / ID Number: | 10.1016/j.chroma.2017.03.025 |
Copyright Information: | © 2017 Elsevier B.V. All rights reserved. |
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