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Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: a fast new approach to selective derivatization techniques

Pravadali-Cekic, S, Jones, A, Kazarian, AA, Paull, B ORCID: 0000-0001-6373-6582, Soliven, A, Ritchie, H, Camenzuli, M, Leung, L, Dennis, GR and Shalliker, RA 2017 , 'Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: a fast new approach to selective derivatization techniques' , Journal of Liquid Chromatography and Related Technologies, vol. 40, no. 2 , pp. 83-92 , doi: 10.1080/10826076.2017.1285312.

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Abstract

Reaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R2 ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously.

Item Type: Article
Authors/Creators:Pravadali-Cekic, S and Jones, A and Kazarian, AA and Paull, B and Soliven, A and Ritchie, H and Camenzuli, M and Leung, L and Dennis, GR and Shalliker, RA
Keywords: amino acids, fluorescamine, fluorescence detection, multiplexed detection, postcolumn derivatization, reaction flow chromatography
Journal or Publication Title: Journal of Liquid Chromatography and Related Technologies
Publisher: Marcel Dekker Inc
ISSN: 1082-6076
DOI / ID Number: 10.1080/10826076.2017.1285312
Copyright Information:

© 2017 Taylor & Francis

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