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Abstract

In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2–2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.

Item Type:	Article
Authors/Creators:	Keremidaeska-Markova, M and Radeva, E and Mitev, D and Hristova-Panusheva, K and Paull, B and Nesterenko, P and Sepitka, J and Junkar, I and Iglic, A and Krasteva, N
Keywords:	detonation nanodiamonds, organosilicone, bone implants, stiffness, cell adhesion and growth
Journal or Publication Title:	Turkish Journal of Biology
Publisher:	Scientific and Technical Research Council of Turkey
ISSN:	1300-0152
DOI / ID Number:	https://doi.org/10.3906/biy-1711-26
Copyright Information:	© TÜBİTAK 2018
Related URLs:	Google Scholar: Paull, B UTAS Profile: Paull, B Google Scholar: Nesterenko, P UTAS Profile: Nesterenko, P
Item Statistics:	View statistics for this item

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