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Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells

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Keremidaeska-Markova, M, Radeva, E, Mitev, D, Hristova-Panusheva, K, Paull, B ORCID: 0000-0001-6373-6582, Nesterenko, P ORCID: 0000-0002-9997-0650, Sepitka, J, Junkar, I, Iglic, A and Krasteva, N 2018 , 'Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells' , Turkish Journal of Biology, vol. 42, no. 2 , pp. 195-203 , doi: 10.3906/biy-1711-26.

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Abstract

In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2–2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.

Item Type: Article
Authors/Creators:Keremidaeska-Markova, M and Radeva, E and Mitev, D and Hristova-Panusheva, K and Paull, B and Nesterenko, P and Sepitka, J and Junkar, I and Iglic, A and Krasteva, N
Keywords: detonation nanodiamonds, organosilicone, bone implants, stiffness, cell adhesion and growth
Journal or Publication Title: Turkish Journal of Biology
Publisher: Scientific and Technical Research Council of Turkey
ISSN: 1300-0152
DOI / ID Number: 10.3906/biy-1711-26
Copyright Information:

© TÜBİTAK 2018

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