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Guard cells in fern stomata are connected by plasmodesmata, but control cytosolic Ca2+ levels autonomously

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Voss, LJ, McAdam, SAM ORCID: 0000-0002-9625-6750, Knoblauch, M, Rathje, JM, Brodribb, TJ ORCID: 0000-0002-4964-6107, Hedrich, R and Roelfsema, MRG 2018 , 'Guard cells in fern stomata are connected by plasmodesmata, but control cytosolic Ca2+ levels autonomously' , New Phytologist, vol. 219, no. 1 , pp. 206-215 , doi: 10.1111/nph.15153.

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Abstract

Recent studies have revealed that some responses of fern stomata to environmental signals differ from those of their relatives in seed plants. However, it is unknown whether the biophysical properties of guard cells differ fundamentally between species of both clades. Intracellular micro-electrodes and the fluorescent Ca2+ reporter FURA2 were used to study voltage-dependent cation channels and Ca2+ signals in guard cells of the ferns Polypodium vulgare and Asplenium scolopendrium. Voltage clamp experiments with fern guard cells revealed similar properties of voltage-dependent K+ channels as found in seed plants. However, fluorescent dyes moved within the fern stomata, from one guard cell to the other, which does not occur in most seed plants. Despite the presence of plasmodesmata, which interconnect fern guard cells, Ca2+ signals could be elicited in each of the cells individually. Based on the common properties of voltage-dependent channels in ferns and seed plants, it is likely that these key transport proteins are conserved in vascular plants. However, the symplastic connections between fern guard cells in mature stomata indicate that the biophysical mechanisms that control stomatal movements differ between ferns and seed plants.

Item Type: Article
Authors/Creators:Voss, LJ and McAdam, SAM and Knoblauch, M and Rathje, JM and Brodribb, TJ and Hedrich, R and Roelfsema, MRG
Keywords: calcium signals, ferns, guard cell, plasmodesmata, potassium channels, seed plants, stomata.
Journal or Publication Title: New Phytologist
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 1469-8137
DOI / ID Number: 10.1111/nph.15153
Copyright Information:

Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/

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