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Hydroxyl radical scavenging by cerium oxide nanoparticles improves Arabidopsis salinity tolerance by enhancing leaf mesophyll potassium retention

Wu, H, Shabala, L ORCID: 0000-0002-5360-8496, Shabala, S ORCID: 0000-0003-2345-8981 and Giraldo, JP 2018 , 'Hydroxyl radical scavenging by cerium oxide nanoparticles improves Arabidopsis salinity tolerance by enhancing leaf mesophyll potassium retention' , Environmental Science: Nano, vol. 5 , pp. 1567-1583 , doi: 10.1039/c8en00323h.

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Abstract

Salinity is a widespread environmental stress that severely limits crop yield worldwide. Cerium oxide nanoparticles (nanoceria) have the unique capability of catalytically reducing levels of stress-induced reactive oxygen species (ROS) including hydroxyl radicals (˙OH) that lack enzymatic scavenging pathways. The underlying mechanisms of how nanoceria ROS scavenging augments plant tolerance to environmental stress are not well understood. Herein, we demonstrate that catalytic ˙OH scavenging by nanoceria in Arabidopsis thaliana leaves significantly improves mesophyll K+ retention, a key trait associated with salinity stress tolerance. Leaves with mesophyll cells interfaced with 50 mg L−1 polyIJacrylic acid) coated nanoceria (PNC) have significantly higher (P P + dye intensity in leaf mesophyll cells relative to controls. Non-invasive microelectrode ion flux electrophysiological (MIFE) measurements indicated that PNC-leaves have about three-fold lower NaCl-induced K+ efflux from leaf mesophyll cells compared to controls upon exposure to salinity stress. The ROS-activated nonselective cation channels (ROS-NSCC) in the plasma membrane of leaf mesophyll cells were identified as the main ˙OH-inducible K+ efflux channels. Long term catalytic scavenging of ˙OH in leaves by PNC enhances plant photosynthetic performance under salinity stress by enabling plasma membrane channels/transporters to coordinately retain higher levels of K+ in the leaf mesophyll cell cytosol. PNC augmented plant ROS scavenging provides a key tool for understanding and improving plant tolerance against abiotic stresses such as salinity.

Item Type: Article
Authors/Creators:Wu, H and Shabala, L and Shabala, S and Giraldo, JP
Keywords: potassium, salinity, mesophyll, ion channels
Journal or Publication Title: Environmental Science: Nano
Publisher: Royal Society of Chemistry
ISSN: 2051-8153
DOI / ID Number: 10.1039/c8en00323h
Copyright Information:

Copyright 2018 The Royal Society of Chemistry

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