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Specific inhibitor of Notch‑3 enhances the sensitivity of NSCLC cells to gemcitabine

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Hu, B-D, Guo, J, Ye, Y ORCID: 0000-0003-0025-8434, Du, T, Cheng, C-S, Jiang, Q, Liu, R-N and Zhang, Y-B 2018 , 'Specific inhibitor of Notch‑3 enhances the sensitivity of NSCLC cells to gemcitabine' , Oncology Reports, vol. 40, no. 1 , pp. 155-164 , doi: 10.3892/or.2018.6448.

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Abstract

Notch‑3 is a receptor of the Notch signaling pathway and plays an important role in regulating self‑renewal, differentiation and apoptosis in cancer cells. Overexpression of Notch‑3 has been proved to be associated with resistance to gemcitabine (GEM) and poor patient prognosis for various malignant tumors. In the present study, two non‑small cell lung cancer (NSCLC) cell lines, H1299 and A549, were induced with GEM for two months and then were treated with various concentrations of a Notch signaling blocker, N‑[N‑(3,5‑difluorophenacetyl)‑L‑alanyl]‑S‑phenylglycine t‑butyl ester (DAPT), with the goal of reducing expression of Notch intracellular domain 3 (NICD3). Both cell lines were subsequently treated with either DAPT or DAPT combined with GEM and then viability, apoptosis, colony formation and cell count assays were performed. DAPT treatment effectively downregulated the expression of NICD3 in both cell lines. DAPT combined with GEM also significantly reduced the percentage of viable cells in both cell lines, while increasing the percentage of apoptotic cells, compared with GEM alone. In the clonogenicity assays, the combination of DAPT and GEM led to a decrease in clone numbers and significantly greater inhibition of the H1299 and A549 cells compared to treatment with DAPT or GEM alone. Meanwhile, levels of the apoptosis‑related proteins, Bcl‑2 and Bax, were found to be affected by the various treatments. Thus Notch‑3 appears to be a promising target for gene therapy and DAPT is able to mediate a strong antitumor effect in NSCLC cells that overexpress Notch‑3. Further studies of a combined treatment regimen with DAPT and GEM are warranted and may provide greater efficacy and safety in the treatment of NSCLC patients.

Item Type: Article
Authors/Creators:Hu, B-D and Guo, J and Ye, Y and Du, T and Cheng, C-S and Jiang, Q and Liu, R-N and Zhang, Y-B
Keywords: non‑small cell lung cancer, Notch signaling, DAPT, gemcitabine, chemoresistance
Journal or Publication Title: Oncology Reports
Publisher: Professor D A Spandidos
ISSN: 1021-335X
DOI / ID Number: 10.3892/or.2018.6448
Copyright Information:

Copyright 2018 The Authors. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/

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