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Abstract

Store-operated Ca2+ entry is a pathway that is remodelled in a variety of cancers, and altered expression of the componentsof store-operated Ca2+ entry is a feature of breast cancer cells of the basal molecular subtype. Studies of store-operated Ca2+entry in breast cancer cells have used non-specifc pharmacological inhibitors, complete depletion of intracellular Ca2+ storesand have mostly focused on MDA-MB-231 cells (a basal B breast cancer cell line). These studies compared the efects of theselective store-operated Ca2+ entry inhibitors Synta66 and YM58483 (also known as BTP2) on global cytosolic free Ca2+([Ca2+]CYT) changes induced by physiological stimuli in a diferent breast cancer basal cell line model, MDA-MB-468. Theefects of these agents on proliferation as well as serum and epidermal growth factor (EGF) induced migration were alsoassessed. Activation with the purinergic receptor activator adenosine triphosphate, produced a sustained increase in [Ca2+]CYTthat was entirely dependent on store-operated Ca2+ entry. The protease activated receptor 2 activator, trypsin, and EGF alsoproduced Ca2+ infux that was sensitive to both Synta66 and YM58483. Serum-activated migration of MDA-MB-468 breastcancer cells was sensitive to both store-operated Ca2+ inhibitors. However, proliferation and EGF-activated migration wasdiferentially afected by Synta66 and YM58483. These studies highlight the need to defne the exact mechanisms of actionof diferent store-operated calcium entry inhibitors and the impact of such diferences in the control of tumour progressionpathways.

Item Type:	Article
Authors/Creators:	Azimi, I and Bong, AH and Poo, GXH and Armitage, K and Lok, D and Roberts-Thomson, SJ and Monteith, GR
Keywords:	Pharmacology, Cancer
Journal or Publication Title:	Cellular and Molecular Life Sciences
Publisher:	Birkhauser Verlag Ag
ISSN:	1420-682X
DOI / ID Number:	10.1007/s00018-018-2904-y
Copyright Information:	© Springer Nature Switzerland AG 2018
Related URLs:	Google Scholar: Azimi, I
Item Statistics:	View statistics for this item

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