Open Access Repository
A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics
Altmetric
Altmetric
ORCID: 0000-0002-3238-426X, Shinde, TS ORCID: 0000-0002-8551-5841, Shastri, MD ORCID: 0000-0002-1012-2779, Perera, APP ORCID: 0000-0002-3674-9940, Tristram, S ORCID: 0000-0002-8485-0322, Martoni, CJ, Gundamaraju, R, Ahuja, KDK ORCID: 0000-0002-0323-4692, Ball, M and Eri, R ORCID: 0000-0003-1688-8043 2018
, 'A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics'
, International Journal of Medical Sciences, vol. 15
, pp. 840-848
, doi: https://doi.org/10.7150/ijms.25004.
|
PDF
126843 - A huma...pdf | Download (941kB) | Preview |
Abstract
Background: The health benefits of probiotics are well established and known to be strain-specific.However, the role of probiotics obtained from different origins and their efficacy largely remainsunexplored. The aim of this study is to investigate the in vitro efficacy of probiotics from differentorigins.Methods: Probiotic strains utilized in this study include Lactobacillus acidophilus DDS-1 (humanorigin), Bifidobacterium animalis ssp. lactis UABla-12 (human origin), L. plantarum UALp-05 (plantorigin) and Streptococcus thermophilus UASt-09 (dairy origin). Screening assays such as in vitrodigestion simulation, adhesion, cell viability and cytokine release were used to evaluate the probioticpotential.Results: All strains showed good resistance in the digestion simulation process, especially DDS-1and UALp-05, which survived up to a range of 107 to 108 CFU/mL from an initial concentration of 109CFU/mL. Two human colonic mucus-secreting cells, HT-29 and LS174T, were used to assess theadhesion capacity, cytotoxicity/viability, and cytokine quantification. All strains exhibited goodadhesion capacity. No significant cellular cytotoxicity or loss in cell viability was observed. DDS-1and UALp-05 significantly upregulated anti-inflammatory IL-10 and downregulated pro-inflammatoryTNF-α cytokine production. All the strains were able to downregulate IL-8 cytokine levels.Conclusion: Of the 4 strains tested, DDS-1 demonstrated superior survival rates, good adhesioncapacity and strong immunomodulatory effect under different experimental conditions.
| Item Type: | Article |
|---|---|
| Authors/Creators: | Vemuri, RC and Shinde, TS and Shastri, MD and Perera, APP and Tristram, S and Martoni, CJ and Gundamaraju, R and Ahuja, KDK and Ball, M and Eri, R |
| Keywords: | probiotics, gut health |
| Journal or Publication Title: | International Journal of Medical Sciences |
| Publisher: | Ivyspring International Publisher |
| ISSN: | 1449-1907 |
| DOI / ID Number: | https://doi.org/10.7150/ijms.25004 |
| Copyright Information: | © Ivyspring International Publisher. Licensed under Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ |
| Related URLs: | |
| Item Statistics: | View statistics for this item |
Actions (login required)
 |
Item Control Page |