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A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics

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Vemuri, R ORCID: 0000-0002-3238-426X, Shinde, T, Shastri, M ORCID: 0000-0002-1012-2779, Perera, AP, Tristram, S ORCID: 0000-0002-8485-0322, Martoni, CJ, Gundamaraju, R, Ahuja, KDK ORCID: 0000-0002-0323-4692, Ball, M and Eri, R ORCID: 0000-0003-1688-8043 2018 , 'A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics' , International Journal of Medical Sciences, vol. 15 , pp. 840-848 , doi: 10.7150/ijms.25004.

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Abstract

Background: The health benefits of probiotics are well established and known to be strain-specific.However, the role of probiotics obtained from different origins and their efficacy largely remainsunexplored. The aim of this study is to investigate the in vitro efficacy of probiotics from differentorigins.Methods: Probiotic strains utilized in this study include Lactobacillus acidophilus DDS-1 (humanorigin), Bifidobacterium animalis ssp. lactis UABla-12 (human origin), L. plantarum UALp-05 (plantorigin) and Streptococcus thermophilus UASt-09 (dairy origin). Screening assays such as in vitrodigestion simulation, adhesion, cell viability and cytokine release were used to evaluate the probioticpotential.Results: All strains showed good resistance in the digestion simulation process, especially DDS-1and UALp-05, which survived up to a range of 107 to 108 CFU/mL from an initial concentration of 109CFU/mL. Two human colonic mucus-secreting cells, HT-29 and LS174T, were used to assess theadhesion capacity, cytotoxicity/viability, and cytokine quantification. All strains exhibited goodadhesion capacity. No significant cellular cytotoxicity or loss in cell viability was observed. DDS-1and UALp-05 significantly upregulated anti-inflammatory IL-10 and downregulated pro-inflammatoryTNF-α cytokine production. All the strains were able to downregulate IL-8 cytokine levels.Conclusion: Of the 4 strains tested, DDS-1 demonstrated superior survival rates, good adhesioncapacity and strong immunomodulatory effect under different experimental conditions.

Item Type: Article
Authors/Creators:Vemuri, R and Shinde, T and Shastri, M and Perera, AP and Tristram, S and Martoni, CJ and Gundamaraju, R and Ahuja, KDK and Ball, M and Eri, R
Keywords: probiotics, gut health
Journal or Publication Title: International Journal of Medical Sciences
Publisher: Ivyspring International Publisher
ISSN: 1449-1907
DOI / ID Number: 10.7150/ijms.25004
Copyright Information:

© Ivyspring International Publisher. Licensed under Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/

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