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Towards complete identification of allergens in Jack Jumper (Myrmecia pilosula) ant venom and their clinical relevance: An immunoproteomic approach

Wanandy, J, Wilson, R ORCID: 0000-0003-0152-4394, Gell, D ORCID: 0000-0003-0382-1181, Rose, HE, Gueven, N ORCID: 0000-0003-3782-767X, Davies, NW ORCID: 0000-0002-9624-0935, Brown, SGA and Wiese, MD 2018 , 'Towards complete identification of allergens in Jack Jumper (Myrmecia pilosula) ant venom and their clinical relevance: An immunoproteomic approach' , Clinical and Experimental Allergy, vol. 48, no. 9 , 1222–1234 , doi: 10.1111/cea.13224.

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Abstract

Background: The venomous stings of Jack Jumper ant (JJA; species of the Myrmeciapilosula taxonomic group) are a significant public health issue in parts of south‐easternand south‐western Australia, causing anaphylaxis in approximately 3% of thepopulation. Three allergenic peptides, Myr p 1, Myr p 2 and Myr p 3, and one histamine‐releasingpeptide, pilosulin 5, have been fully described, but there are at least5 additional high molecular weight IgE‐binding components that have not been identified.Objective: To identify IgE‐binding components in JJA venom (JJAV) and to relatethe IgE recognition of these components to relevant clinical parameters.Methods: Identification of IgE‐binding components and determination of their sensitizingprevalence was performed using SDS‐PAGE immunoblot assay and sera from90 patients with confirmed allergy to JJAV. Tandem mass spectrometry was usedfor identification of novel JJAV components fractionated by size exclusion chromatography(SEC) and SDS‐PAGE.Results: Using SDS‐PAGE immunoblot, 10 IgE‐binding bands were identified inJJAV, two of which were recognized by 81% and 47% of the population studied.Mass spectrometry identified 17 novel JJAV proteins, including 2 glycoproteins, andconfirmed the presence of 4 known Myr p and pilosulin peptides in JJAV. Most ofthe newly identified IgE‐binding proteins were enzymes, including phospholipase A2,hyaluronidase, arginine kinase and dipeptidyl peptidase IV. Correlations were foundbetween recognition of certain IgE‐binding bands with JJAV‐specific IgE titre byImmunoCAP, intradermal test threshold and treatment‐related issues.Conclusions and Clinical Relevance: This study has for the first time revealed theidentity of various proteins with IgE‐binding capacity in the venom of JJA anddemonstrated their clinical relevance in the diagnosis and treatment of JJAV allergy.

Item Type: Article
Authors/Creators:Wanandy, J and Wilson, R and Gell, D and Rose, HE and Gueven, N and Davies, NW and Brown, SGA and Wiese, MD
Keywords: allergen, Hymenoptera venom, immunoproteomics, Myrmecia pilosula
Journal or Publication Title: Clinical and Experimental Allergy
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 1365-2222
DOI / ID Number: 10.1111/cea.13224
Copyright Information:

Copyright 2018 John Wiley & Sons Ltd

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