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Development of microsatellites using next-generation sequencing for Acacia crassicarpa

McKinnon, GE, Larcombe, MJ, Griffin, AR and Vaillancourt, RE ORCID: 0000-0002-1159-9149 2018 , 'Development of microsatellites using next-generation sequencing for Acacia crassicarpa' , Journal of Tropical Forest Science, vol. 30, no. 2 , pp. 252-258 , doi: 10.26525/jtfs2018.30.2.252258.

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Abstract

Acacia crassicarpa is a tropical forestry species that is increasingly being planted in South-East Asia. Usingnext- generation sequencing, we developed 12 nuclear microsatellite markers for A. crassicarpa and optimisedthem for assay in three multiplex sets. We tested the 12 loci on 34 A. crassicarpa samples and found thatpolymorphism ranged from 4 to 8 alleles per locus (average = 5.8). The loci were easy to score in terms ofbinning and their reproducibility and polymerase chain reaction success rate were high. Given their usabilityand polymorphism we believe that these 12 loci will be useful for DNA profiling and mating system analysis withdirect application in breeding programmes and the conservation of wild populations. Six of the markers alsoamplified products in related A. mangium and A. auriculiformis, with four being polymorphic in three samplesof each species. Therefore, these markers added more broadly to the genomic resources available in Acacia.

Item Type: Article
Authors/Creators:McKinnon, GE and Larcombe, MJ and Griffin, AR and Vaillancourt, RE
Keywords: disease resistance, DNA profiling, genetic improvement, simple sequence repeats (SSR), multiplex microsatellites, Acacia silviculture, genetic resources
Journal or Publication Title: Journal of Tropical Forest Science
Publisher: Forest Research Inst Malaysia
ISSN: 0128-1283
DOI / ID Number: 10.26525/jtfs2018.30.2.252258
Copyright Information:

Copyright 2018 Forest Research Institute Malaysia

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