Open Access Repository
A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis

Full text not available from this repository.
Abstract
A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.
Item Type: | Article |
---|---|
Authors/Creators: | Quirino, JP |
Keywords: | capillary electrophoresis, cationic cyclodextrins, dynamic coating, neutral surfaces, proteins |
Journal or Publication Title: | Journal of Separation Science |
Publisher: | Wiley-V C H Verlag Gmbh |
ISSN: | 1615-9314 |
DOI / ID Number: | 10.1002/jssc.201700610 |
Copyright Information: | Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
Related URLs: | |
Item Statistics: | View statistics for this item |
Actions (login required)
![]() |
Item Control Page |