Full text not available from this repository.

Abstract

A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.

Item Type:	Article
Authors/Creators:	Quirino, JP
Keywords:	capillary electrophoresis, cationic cyclodextrins, dynamic coating, neutral surfaces, proteins
Journal or Publication Title:	Journal of Separation Science
Publisher:	Wiley-V C H Verlag Gmbh
ISSN:	1615-9314
DOI / ID Number:	10.1002/jssc.201700610
Copyright Information:	Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Related URLs:	Google Scholar: Quirino, JP UTAS Profile: Quirino, JP
Item Statistics:	View statistics for this item

Actions (login required)

Item Control Page