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A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis

Quirino, JP ORCID: 0000-0002-8275-9672 2017 , 'A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis' , Journal of Separation Science, vol. 40, no. 24 , pp. 4835-4838 , doi: 10.1002/jssc.201700610.

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Abstract

A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.

Item Type: Article
Authors/Creators:Quirino, JP
Keywords: capillary electrophoresis, cationic cyclodextrins, dynamic coating, neutral surfaces, proteins
Journal or Publication Title: Journal of Separation Science
Publisher: Wiley-V C H Verlag Gmbh
ISSN: 1615-9314
DOI / ID Number: 10.1002/jssc.201700610
Copyright Information:

Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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