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Thread based electrofluidic platform for direct metabolite analysis in complex samples

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Cabot, JM ORCID: 0000-0002-3305-078X, Breadmore, MC ORCID: 0000-0001-5591-4326 and Paull, B ORCID: 0000-0001-6373-6582 2018 , 'Thread based electrofluidic platform for direct metabolite analysis in complex samples' , Analytica Chimica Acta, vol. 1000 , pp. 283-292 , doi: 10.1016/j.aca.2017.10.029.

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Abstract

The application of electrophoresis upon commercial threads is investigated for development of low-cost diagnostics assays, designed for the matrix separation and quantification of low abundance metabolites in complex samples – in this work riboflavin in human urine. Zone electrophoresis was evaluated upon 8 commercially available threads, with several synthetic threads exhibiting higher electroosmotic flow (EOF) and increased electrophoretic mobility of the rhodamine 6G, rhodamine B, and fluorescein. Of those tested, a nylon bundle was selected as the best platform, offering less band dispersion and higher resolution, a high relative EOF, whilst minimising the contribution of joule heating. A novel 3D printed platform was designed, based on a modular system, facilitating the electrophoresis process and rapid assembly, whilst offering the potential for multiplexed analysis or investigation of more complex systems. Using the thread-based electrophoresis system, riboflavin was determined in less than 2 minutes. The device exhibited a linear working range from 0.1 to 15 µg/mL of riboflavin in urine, and was in good agreement with capillary electrophoresis measurements.

Item Type: Article
Authors/Creators:Cabot, JM and Breadmore, MC and Paull, B
Keywords: electrofluidics, thread-based assays, metabolite analysis
Journal or Publication Title: Analytica Chimica Acta
Publisher: Elsevier Science Bv
ISSN: 0003-2670
DOI / ID Number: 10.1016/j.aca.2017.10.029
Copyright Information:

Copyright 2017 Elsevier B.V.

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