Open Access Repository

Plasma proteomics reveals gestational age-specific responses to mechanical ventilation and identifies the mechanistic pathways that initiate preterm lung injury

Downloads

Downloads per month over past year

Pereira-Fantini, PM, Byars, SG, McCall, KE, Perkins, EJ, Oakley, RB, Dellaca, RL, Dargaville, PA, Davis, PG, Ignjatovic, V and Tingay, DG 2018 , 'Plasma proteomics reveals gestational age-specific responses to mechanical ventilation and identifies the mechanistic pathways that initiate preterm lung injury' , Scientific Reports, vol. 8, no. 1 , pp. 1-13 , doi: 10.1038/s41598-018-30868-x.

[img]
Preview
PDF
128139 Journal ...pdf | Download (4MB)

| Preview

Abstract

The preterm lung is particularly vulnerable to ventilator-induced lung injury (VILI) as a result of mechanical ventilation. However the developmental and pathological cellular mechanisms influencing the changing patterns of VILI have not been comprehensively delineated, preventing the advancement of targeted lung protective therapies. This study aimed to use SWATH-MS to comprehensively map the plasma proteome alterations associated with the initiation of VILI following 60 minutes of standardized mechanical ventilation from birth in three distinctly different developmental lung states; the extremely preterm, preterm and term lung using the ventilated lamb model. Across these gestations, 34 proteins were differentially altered in matched plasma samples taken at birth and 60 minutes. Multivariate analysis of the plasma proteomes confirmed a gestation-specific response to mechanical ventilation with 79% of differentially-expressed proteins altered in a single gestation group only. Six cellular and molecular functions and two physiological functions were uniquely enriched in either the extremely preterm or preterm group. Correlation analysis supported gestation-specific protein-function associations within each group. In identifying the gestation-specific proteome and functional responses to ventilation we provide the founding evidence required for the potential development of individualized respiratory support approaches tailored to both the developmental and pathological state of the lung.

Item Type: Article
Authors/Creators:Pereira-Fantini, PM and Byars, SG and McCall, KE and Perkins, EJ and Oakley, RB and Dellaca, RL and Dargaville, PA and Davis, PG and Ignjatovic, V and Tingay, DG
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
DOI / ID Number: 10.1038/s41598-018-30868-x
Copyright Information:

Copyright 2018 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

Related URLs:
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page
TOP