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Measurement duration affects the calculation of whole body protein turnover kinetics but not between-day variability

Ruddick-Collins, LC, King, NA and Byrne, NM ORCID: 0000-0001-5310-6640 2018 , 'Measurement duration affects the calculation of whole body protein turnover kinetics but not between-day variability' , Metabolism: clinical and experimental, vol. 87 , pp. 80-86 , doi: 10.1016/j.metabol.2018.06.003.

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Background:Assessment of whole body protein turnover (WBPT) can provide fundamental information about protein kinetics which underpins the conservation of lean tissue. Reliability and methodology studies on the measurement of WBPT are scarce. This study aimed to assess the effects of urine collection duration (9 versus 12 h) and the reproducibility of WBPT with the end product method calculated from ammonia as the end product.Methods:WBPT was assessed in 21 healthy participants (11M, 10F) on 2 test days. WBPT was assessed using the end product method with a single dose of 15N glycine with ammonia as end product in a postprandial state with 9 and 12-h urine collections.Results:The CV for protein flux averaged 10% and 12% for 9 and 12-h urine collections respectively. Protein flux, synthesis and balance were significantly higher and protein breakdown significantly lower with 9-h urine collections compared to 12-h collections (P  0.962, P Conclusions:The reproducibility of WBPT with ammonia as the end product was similar to previously reported reproducibility of the gold standard precursor technique. The use of a 12-h urine collection is more effective to achieve full turnover of the ammonia free amino acid (AA) pool.

Item Type: Article
Authors/Creators:Ruddick-Collins, LC and King, NA and Byrne, NM
Keywords: whole body protein turnover, end product method, measurement duration, reproducibility, variability<sup>15</sup>N, glycine
Journal or Publication Title: Metabolism: clinical and experimental
Publisher: Grune & Stratton
ISSN: 0026-0495
DOI / ID Number: 10.1016/j.metabol.2018.06.003
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Copyright 2018 Elsevier Inc.

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