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Abstract

Human monocytes and dendritic cells express transient receptor potential vanilloid 1 (TRPV1) which may play a role in mediating the inflammatory, immune and cancer surveillance responses of these cells. The aim of the present study was to investigate TRPV1 expression and function in THP-1 monocytic cells. RT-PCR and Western blot were used to detect TRPV1. The metabolic activity and viability of THP-1 cells following exposure to vanilloids was assessed using resorufin production from rezazurin. Cytokine release was measured using ELISA. TRPV1 was expressed in cultured THP-1 monocytic cells and naïve monocytes. Lower concentrations (250 M, all agonists decreased metabolic activity. Capsaicin-stimulated THP-1 metabolic activity was blocked by the TRPV1 antagonist, 5-iodo-resiniferatoxin (5’-IRTX), whereas the decline in resorufin production by THP-1 cells at higher capsaicin concentrations (due to cell death), was not affected by 5’-IRTX. Finally, capsaicin (125 M) significantly increased lipopolysaccharide-stimulated IL-6 and TNF- release from THP-1 cells, whereas phytohaemagglutinin-stimulated IL-1, TNF-, MCP-1 and IL-6 release were concentration-dependently inhibited by capsaicin. Modulation of IL-1release was not TRPV1 mediated. Overall, these results show that functional TRPV1 channels are present in naïve monocytes and THP-1 cells, and when activated, increase cell metabolic activity. In addition, capsaicin modifies cytokine release from THP-1 cells and induces cell death, most likely by a mechanism that is independent of TRPV1 activation.

Item Type:	Article
Authors/Creators:	Kunde, DA and Yingchoncharoen, J and Jurkovic, S and Geraghty, DP
Keywords:	TRPV1; capsaicin; cytokines; immunomodulation; monocytes
Journal or Publication Title:	Toxicology and Applied Pharmacology
Publisher:	Academic Press Inc Elsevier Science
ISSN:	0041-008X
DOI / ID Number:	10.1016/j.taap.2018.09.025
Copyright Information:	© 2018 Elsevier Inc.
Related URLs:	UTAS Profile: Kunde, DA Google Scholar: Geraghty, DP UTAS Profile: Geraghty, DP
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