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A cost-effective technique for generating preservable biomass smoke extract and measuring its effect on cell receptor expression in human bronchial epithelial cells

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Rajendra, KC, Zosky, GR ORCID: 0000-0001-9039-0302, Shukla, SD and O'Toole, RF ORCID: 0000-0002-4579-4479 2018 , 'A cost-effective technique for generating preservable biomass smoke extract and measuring its effect on cell receptor expression in human bronchial epithelial cells' , Biology Methods and Protocols, vol. 3, no. 1 , pp. 1-9 , doi: 10.1093/biomethods/bpy010.

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Abstract

Nearly half of the world’s population uses biomass fuel for the purposes of cooking and heating. Smoke derived from biomassincreases the risk of the development of lung diseases, including pneumonia, chronic obstructive pulmonary disease,airway tract infections, and lung cancer. Despite the evidence linking biomass smoke exposure to pulmonary disease, onlya small number of experimental studies have been conducted on the impact of biomass smoke on airway epithelial cells.This is in part due to the lack of a standard and easily accessible procedure for the preparation of biomass smoke. Here, wedescribe a cost-effective and reproducible method for the generation of different smoke extracts, in particular, cow dungsmoke extract (CDSE) and wood smoke extract (WSE) for use in a range of biological applications. We examined the effect ofthe biomass smoke extracts on human bronchial epithelial cell expression of a known responder to cigarette smoke exposure(CSE), the platelet-activating factor receptor (PAFR). Similar to the treatment with CSE, we observed a dose-dependentincrease in PAFR expression on human airway epithelial cells that were exposed to CDSE and WSE. This method providesbiomass smoke in a re-usable form for cell and molecular bioscience studies on the pathogenesis of chronic lung disease.

Item Type: Article
Authors/Creators:Rajendra, KC and Zosky, GR and Shukla, SD and O'Toole, RF
Keywords: biomass smoke, cigarette smoke extract, chronic obstructive pulmonary disease, platelet-activating factor receptor, non-typeable haemophilus influenzae
Journal or Publication Title: Biology Methods and Protocols
Publisher: Oxford University Press
ISSN: 2396-8923
DOI / ID Number: 10.1093/biomethods/bpy010
Copyright Information:

Copyright 2018 The Author(s) 2018. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/

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