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Single-cell profiling identifies key pathways expressed by iPSCs cultured in different commercial media


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Daniszewski, M, Nguyen, Q, Chy, HS, Singh, V ORCID: 0000-0003-4847-3643, Crombie, DE, Kulkarni, T, Liang, HH, Sivakumaran, P, Lidgerwood, GE, Hernandez, D, Conquest, A, Rooney, LA, Chevalier, S, Andersen, SB, Senabouth, A, Vickers, JC ORCID: 0000-0001-5671-4879, Mackey, DA, Craig, JE, Laslett, AL, Hewitt, AW ORCID: 0000-0002-5123-5999, Powell, JE and Pebay, A 2018 , 'Single-cell profiling identifies key pathways expressed by iPSCs cultured in different commercial media' , iScience, vol. 7 , pp. 30-39 , doi: 10.1016/j.isci.2018.08.016.

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We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.

Item Type: Article
Authors/Creators:Daniszewski, M and Nguyen, Q and Chy, HS and Singh, V and Crombie, DE and Kulkarni, T and Liang, HH and Sivakumaran, P and Lidgerwood, GE and Hernandez, D and Conquest, A and Rooney, LA and Chevalier, S and Andersen, SB and Senabouth, A and Vickers, JC and Mackey, DA and Craig, JE and Laslett, AL and Hewitt, AW and Powell, JE and Pebay, A
Keywords: automation, stem cells research, transcriptomics
Journal or Publication Title: iScience
Publisher: Cell Press
ISSN: 2589-0042
DOI / ID Number: 10.1016/j.isci.2018.08.016
Copyright Information:

Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

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