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Propagation and Purification of Ectromelia Virus

Chaudhri, G, Kaladimou, G, Pandey, P and Karupiah, G ORCID: 0000-0001-6306-4405 2018 , 'Propagation and Purification of Ectromelia Virus' , Current Protocols in Microbiology, vol. 51, no. 1 , doi: 10.1002/cpmc.65.

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Abstract

Ectromelia virus (ECTV) is an orthopoxvirus that causes mousepox in mice. Members of the genus orthopoxvirus are closely related and include variola (the causative agent of smallpox in humans), monkeypox, and vaccinia. Common features of variola virus and ECTV further include a restricted host range and similar disease progression in their respective hosts. Mousepox makes an excellent small animal model for smallpox to investigate pathogenesis, vaccine and antiviral agent testing, host-virus interactions, and immune and inflammatory responses. The availability of a wide variety of inbred, congenic, and gene-knockout mice allows detailed analyses of the host response. ECTV mutant viruses lacking one or more genes encoding immunomodulatory proteins are being used in numerous studies in conjunction with wild-type or gene-knockout mice to study the functions of these genes in host-virus interactions. The methods used for propagation of ECTV in cell culture, purification, and quantification of infectious particles through viral plaque assay are described.

Item Type: Article
Authors/Creators:Chaudhri, G and Kaladimou, G and Pandey, P and Karupiah, G
Journal or Publication Title: Current Protocols in Microbiology
Publisher: John Wiley
ISSN: 1934-8525
DOI / ID Number: 10.1002/cpmc.65
Copyright Information:

Copyright 2018 John Wiley & Sons, Inc.

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