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Abstract

Low translational yield for stroke may reflect the focus of discovery science on rodents rather than humans. Just how little is known about human neuronal ischaemic responses is confirmed by systematic review and meta-analysis revealing that data for the most commonly used SH-SY5Y human cells comprises only 84 papers. Oxygen-glucose deprivation, H2O2, hypoxia, glucose-deprivation and glutamate excitotoxicity yielded − 58, − 61, − 29, − 45 and − 49% injury, respectively, with a dose-response relationship found only for H2O2 injury (R2 = 29.29%, p I2 = 99.36%, df = 132, p R2 = 44.77%, p R2 = 28.64%, p R2 = 4.13%, p p 2O2 injury reported only improvement. In studies using glucose deprivation, intervention generally worsened outcome. There was insufficient data to rank individual interventions, but of the studies reporting greatest improvement (> 90% effect size), 7/13 were of herbal medicine constituents (24.85% of the intervention dataset). We conclude that surprisingly little is known of the human neuronal response to ischaemic injury, and that the large impact of methodology on outcome indicates that further model validation is required. Lack of evidence for randomisation, blinding or power analysis suggests that the intervention data is at substantial risk of bias.

Item Type:	Article
Authors/Creators:	Liu, Y and Eaton, ED and Wills, TE and McCann, SK and Antonic, A and Howells, DW
Keywords:	human ischaemic cascade, SH-SY5Y cells, systematic review and meta-analysis, in vitro ischaemia-related injuries, study quality
Journal or Publication Title:	Translational Stroke Research
Publisher:	Springer
ISSN:	1868-4483
DOI / ID Number:	10.1007/s12975-018-0620-4
Copyright Information:	Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Related URLs:	UTAS Profile: Howells, DW
Item Statistics:	View statistics for this item

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