Open Access Repository

Pregnancy protects against the pro-inflammatory respiratory responses induced by particulate matter exposure

Thaver, S, Bennett, EJ ORCID: 0000-0002-9818-827X, Foa, L ORCID: 0000-0003-4131-8341, Richards, SM ORCID: 0000-0002-5988-0423, Lyons, AB ORCID: 0000-0002-8508-5853 and Zosky, GR ORCID: 0000-0001-9039-0302 2019 , 'Pregnancy protects against the pro-inflammatory respiratory responses induced by particulate matter exposure' , Chemosphere, vol. 225 , pp. 796-802 , doi: 10.1016/j.chemosphere.2019.03.088.

Full text not available from this repository.


Background: Little is known about the effect of pregnancy on the response to particulate matter. The aim of this study was to determine if pregnancy increases the susceptibility to PM from different sources using a mouse model.Methods: Pregnant, eight-week-old C57BL/6J mice were exposed intranasally to 50 μg of diesel exhaust particles (DEP), iron oxide (Fe2O3) or silica (SiO2) in 50 μL of saline, or saline alone, on gestational day (E)7.5, E12.5 and E17.5. Groups of non-pregnant mice were exposed on day (D)0, D5 and D10. Biological samples were collected 24 h after the last exposure. Serum IL-4 and IL-6 levels were quantified by ELISA. Bronchoalveolar lavage (BAL) fluid was collected for inflammatory cells counts and assessment of IFN-ɣ, IL-4, IL-5, IL-6, IL-8 and IL-10 levels by ELISA. The spleen and thymus were also collected and the percentage of B cells and CD4+, CD8+ and CD4+CD25 + T cells were determined by flow cytometry.Results: Exposure to silica caused an influx of lymphocytes, eosinophils and neutrophils into the lung. The magnitude of this response was suppressed by pregnancy. Pregnancy also enhanced the production of CD4+CD25 + T cells in response to DEP and silica exposure.Conclusions: Collectively, our data suggest that pregnancy reduces the inflammatory response to silica and alters the immune response to DEP. These responses were accompanied by pregnancy related changes including increased IL-4 production, reduced IL-8 production and an increase in the proportion of CD4+CD25 + T cells in response to PM exposure.

Item Type: Article
Authors/Creators:Thaver, S and Bennett, EJ and Foa, L and Richards, SM and Lyons, AB and Zosky, GR
Keywords: pregnancy, particulate matter, inflammation, immune cells
Journal or Publication Title: Chemosphere
Publisher: Pergamon-Elsevier Science Ltd
ISSN: 0045-6535
DOI / ID Number: 10.1016/j.chemosphere.2019.03.088
Copyright Information:

© 2019 Elsevier Ltd

Related URLs:
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page