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Generation of vestibular tissue-like organoids from human pluripotent stem cells using the rotary cell culture system

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Mattei, C, Lim, R, Drury, H, Nasr, B, Li, Z, Tadros, MA, D'Abaco, GM, Stok, KS, Nayagam, BA and Dottori, M 2019 , 'Generation of vestibular tissue-like organoids from human pluripotent stem cells using the rotary cell culture system' , Frontiers in Cell and Developmental Biology, vol. 7 , doi: 10.3389/fcell.2019.00025.

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Abstract

Hair cells are specialized mechanosensitive cells responsible for mediating balance and hearing within the inner ear. In mammals, hair cells are limited in number and do not regenerate. Human pluripotent stem cells (hPSCs) provide a valuable source for deriving human hair cells to study their development and design therapies to treat and/or prevent their degeneration. In this study we used a dynamic 3D Rotary Cell Culture System (RCCS) for deriving inner ear organoids from hPSCs. We show RCCS-derived organoids recapitulate stages of inner ear development and give rise to an enriched population of hair cells displaying vestibular-like morphological and physiological phenotypes, which resemble developing human fetal inner ear hair cells as well as the presence of accessory otoconia-like structures. These results show that hPSC-derived organoids can generate complex inner ear structural features and be a resource to study inner ear development.

Item Type: Article
Authors/Creators:Mattei, C and Lim, R and Drury, H and Nasr, B and Li, Z and Tadros, MA and D'Abaco, GM and Stok, KS and Nayagam, BA and Dottori, M
Keywords: vestibular hair cells, human pluripotent stem cells, organoids, human fetal tissue, inner ear
Journal or Publication Title: Frontiers in Cell and Developmental Biology
Publisher: Frontiers Research Foundation
ISSN: 2296-634X
DOI / ID Number: 10.3389/fcell.2019.00025
Copyright Information:

Copyright 2019 Mattei, Lim, Drury, Nasr, Li, Tadro, D'Abaco, Stok, Nayagam and Dottori. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

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