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Abstract

Interleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α,25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3' untranslated region (3'UTR) of IL-34 mRNA. Intriguingly, using qPCR we demonstrated that miR-31 levels were negatively correlated to IL-34 mRNA levels in different cell lines. By examining the effect of miR-31 on IL-34 3' UTR reporter luciferase activity and on IL-34 mRNA and argonaute RISC catalytic component 2 (AGO2) binding, it was found that miR-31 bound directly to IL-34 3'UTR and regulated the post-transcriptional expression of IL-34 in MGC-803 cells. Moreover, a miR-31 mimic significantly reduced IL-34 expression levels while a miR-31 inhibitor up-regulated IL-34 expression in KYSE-45 and HT-29 cells. Taken together, these results show that miR-31 negatively regulates IL-34 expression by directly binding to the IL-34 3' UTR in vitro.

Item Type:	Article
Authors/Creators:	Li, M and Dong, Y and Chen, Z and Meng, L and Liu, X and Zhang, X and Wang, H and Mao, W and Zhang, J and Jiang, Z and Huang, T and Hu, J and Luo, P and Korner, H and Ying, S and Li, J
Keywords:	IL-34, MiR-31, MicroRNA, regulation
Journal or Publication Title:	Immunological Investigations
Publisher:	Marcel Dekker Inc
ISSN:	0882-0139
DOI / ID Number:	10.1080/08820139.2019.1578230
Copyright Information:	Copyright 2019 Taylor & Francis Group, LLC
Related URLs:	Google Scholar: Korner, H UTAS Profile: Korner, H
Item Statistics:	View statistics for this item

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