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Abstract

Decellularized tissue matrices are promising substrates for tissue generation by stem cells to replace poorly regenerating tissues such as cartilage. However, the dense matrix of decellularized cartilage impedes colonisation by stem cells. Here, we show that digestion of elastin fibre bundles traversing auricular cartilage creates channels through which cells can migrate into the matrix. Human chondrocytes and bone marrow-derived mesenchymal stromal cells efficiently colonise elastin-treated scaffolds through these channels, restoring a glycosaminoglycan-rich matrix and improving mechanical properties while maintaining size and shape of the restored tissue. The scaffolds are also rapidly colonised by endogenous cartilage-forming cells in a subcutaneously implanted osteochondral biopsy model. Creating channels for cells in tissue matrices may be a broadly applicable strategy for recellularization and restoration of tissue function.

Item Type:	Article
Authors/Creators:	Lehmann, J and Nurnberger, S and Narcisi, R and Stok, KS and van der Eerden, BCJ and Koevoet, WJLM and Kops, N and ten Berge, D and van Osch, GJ
Keywords:	cartilage tissue engineering, recellularization, auricular, elastin, osteochondral defect
Journal or Publication Title:	Biofabrication
Publisher:	Institute of Physics Publishing Ltd.
ISSN:	1758-5090
DOI / ID Number:	10.1088/1758-5090/ab1436
Copyright Information:	©2019 IOP Publishing Ltd
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