Open Access Repository

A single-cell transcriptome atlas of the adult human retina

Lukowski, SW, Lo, CY, Sharov, AA, Nguyen, Q, Fang, L, Hung, SSC, Zhu, L, Zhang, T, Grunert, U, Nguyen, T, Senabouth, A, Jabbari, JS, Welby, E, Sowden, JC, Waugh, HS, Mackey, A, Pollock, G, Lamb, TD, Wang, P-Y, Hewitt, AW ORCID: 0000-0002-5123-5999, Gillies, MC, Powell, JE and Wong, RCB 2019 , 'A single-cell transcriptome atlas of the adult human retina' , The EMBO Journal , pp. 1-15 , doi: 10.15252/embj.2018100811.

Full text not available from this repository.


The retina is a specialized neural tissue that senses light and initiates image processing. Although the functional organization of specific retina cells has been well studied, the molecular profile of many cell types remains unclear in humans. To comprehensively profile the human retina, we performed single-cell RNA sequencing on 20,009 cells from three donors and compiled a reference transcriptome atlas. Using unsupervised clustering analysis, we identified 18 transcriptionally distinct cell populations representing all known neural retinal cells: rod photoreceptors, cone photoreceptors, Müller glia, bipolar cells, amacrine cells, retinal ganglion cells, horizontal cells, astrocytes, and microglia. Our data captured molecular profiles for healthy and putative early degenerating rod photoreceptors, and revealed the loss of MALAT1 expression with longer post-mortem time, which potentially suggested a novel role of MALAT1 in rod photoreceptor degeneration. We have demonstrated the use of this retina transcriptome atlas to benchmark pluripotent stem cell-derived cone photoreceptors and an adult Müller glia cell line. This work provides an important reference with unprecedented insights into the transcriptional landscape of human retinal cells, which is fundamental to understanding retinal biology and disease.

Item Type: Article
Authors/Creators:Lukowski, SW and Lo, CY and Sharov, AA and Nguyen, Q and Fang, L and Hung, SSC and Zhu, L and Zhang, T and Grunert, U and Nguyen, T and Senabouth, A and Jabbari, JS and Welby, E and Sowden, JC and Waugh, HS and Mackey, A and Pollock, G and Lamb, TD and Wang, P-Y and Hewitt, AW and Gillies, MC and Powell, JE and Wong, RCB
Keywords: photoreceptor subtypes, retina, single-cell RNA sequencing, transcriptome
Journal or Publication Title: The EMBO Journal
Publisher: Oxford Univ Press
ISSN: 0261-4189
DOI / ID Number: 10.15252/embj.2018100811
Copyright Information:

Copyright 2019 The Authors

Related URLs:
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page