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Enhanced osteogenic differentiation of human fetal cartilage rudiment cells on graphene oxide-PLGA hybrid microparticles

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Thickett, SC ORCID: 0000-0002-8168-3856, Hamilton, E, Yogeswaran, G, Zetterlund, PB, Farrugia, BL and Lord, MS 2019 , 'Enhanced osteogenic differentiation of human fetal cartilage rudiment cells on graphene oxide-PLGA hybrid microparticles' , Journal of Functional Biomaterials, vol. 10, no. 3 , pp. 1-12 , doi: 10.3390/jfb10030033.

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Abstract

Poly(d,l–lactide–co–glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration. Therefore, approaches that incorporate osteoconductive molecules are of great interest. Graphene oxide (GO) is gaining popularity for biomedical applications due to its ability to bind biological molecules and present them for enhanced bioactivity. This study reports the preparation of PLGA microparticles via Pickering emulsification using GO as the sole surfactant, which resulted in hybrid microparticles in the size range of 1.1 to 2.4 µm based on the ratio of GO to PLGA in the reaction. Furthermore, this study demonstrated that the hybrid GO-PLGA microparticles were not cytotoxic to either primary human fetal cartilage rudiment cells or the human osteoblast-like cell line, Saos-2. Additionally, the GO-PLGA microparticles promoted the osteogenic differentiation of the human fetal cartilage rudiment cells in the absence of exogenous growth factors to a greater extent than PLGA alone. These findings demonstrate that GO-PLGA microparticles are cytocompatible, osteoinductive and have potential as substrates for bone tissue engineering.

Item Type: Article
Authors/Creators:Thickett, SC and Hamilton, E and Yogeswaran, G and Zetterlund, PB and Farrugia, BL and Lord, MS
Keywords: graphe oxide, microparticles, bone regeneration, PLGA
Journal or Publication Title: Journal of Functional Biomaterials
Publisher: MDPI AG
ISSN: 2079-4983
DOI / ID Number: 10.3390/jfb10030033
Copyright Information:

Copyright 2019 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

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