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Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment

Bindal, P, Gnanasegaran, N, Bindal, U, Haque, N, Ramasamy, TS, Chai, WL and Kasim, NHA 2019 , 'Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment' , Clinical Oral Investigations , pp. 1-11 , doi: 10.1007/s00784-019-02811-5.

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Objective: In this study, we aimed to determine the suitable concentrations of human platelet lysate (HPL) and platelet-richplasma (PRP) for maintaining the in vitro proliferative and angiogenic potential of inflamed dental pulp stem cells.Materials and methods: (LPS)-induced inflamed dental pulp-derived stem cells (iDPSCs) were treated withdifferent concentrations of HPL and PRP (10% and 20%) followed by determination of viability using Alamar Blue assay.Expression of angiogenesis-, adhesion-, and inflammation-regulating genes was also analyzed using RT-qPCR array.Furthermore, expression of growth factors at protein level in the cell culture microenvironment was measured using multiplex assay.Results: Viability of iDPSCs was significantly (p Conclusion: This study suggests that 20% HPL could be optimum to stimulate angiogenesis-related factors in iDPSCs whilemaintaining their viability.Clinical relevance: This data may suggest the potential use of 20% HPL for expanding DPSCs scheduled for clinical trials forregenerative therapies including dental pulp regeneration.

Item Type: Article
Authors/Creators:Bindal, P and Gnanasegaran, N and Bindal, U and Haque, N and Ramasamy, TS and Chai, WL and Kasim, NHA
Keywords: cytokines, growth factors, platelet lysate, platelet-rich plasma, viability
Journal or Publication Title: Clinical Oral Investigations
Publisher: Springer
ISSN: 1432-6981
DOI / ID Number: 10.1007/s00784-019-02811-5
Copyright Information:

Copyright Springer-Verlag GmbH Germany, part of Springer Nature 2019

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