Open Access Repository

Effect of shearing stress on the radial heterogeneity and chromatographic performance of styrene-based polymerised high internal phase emulsions prepared in capillary format

Downloads

Downloads per month over past year

Desire, CT, Arrua, RD, Mansour, FR, Bon, SAF and Hilder, EF 2019 , 'Effect of shearing stress on the radial heterogeneity and chromatographic performance of styrene-based polymerised high internal phase emulsions prepared in capillary format' , RSC Advances, vol. 9, no. 13 , pp. 7301-7313 , doi: 10.1039/C8RA06188B.

[img]
Preview
PDF (Final published version)
135046 - Effect...pdf | Download (4MB)

| Preview

Abstract

Poly(styrene-co-divinylbenzene) monoliths were prepared from the polymerisation of water-in-monomer high internal phase emulsions consisting of a 90 vol% internal phase and stabilised by the non-ionic surfactant Span 80®. The materials were prepared in capillary housings of various internal diameters ranging from 150 μm to 540 μm by simply passing the emulsion through the capillaries. When low shear (300 rpm) was used for emulsification, the droplet and resulting void size distributions were observed to shift towards lower values when the emulsions were forced through capillaries of internal diameter less than 540 μm and all columns exhibited significant radial heterogeneity. When high shear was employed (14 000 rpm) the resulting emulsions preserved their structure when forced through these capillaries and possessed narrower void size distributions with no obvious radial heterogeneity observed upon curing. This resulted in significantly improved chromatographic performance for the separation of a standard mixture of proteins when compared to the materials prepared under low shear.

Item Type: Article
Authors/Creators:Desire, CT and Arrua, RD and Mansour, FR and Bon, SAF and Hilder, EF
Journal or Publication Title: RSC Advances
Publisher: Royal Society of Chemistry
ISSN: 2046-2069
DOI / ID Number: 10.1039/C8RA06188B
Copyright Information:

This journal is © The Royal Society of Chemistry 2019

Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page
TOP