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Identification of a Siglec-F+ granulocyte-macrophage progenitor

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Bolden, JE, Lucas, EC, Zhou, G, O'Sullivan, JA, de Graaf, CA, McKenzie, MD, Di Rago, L, Baldwin, TM, Shortt, J, Alexander, WS, Bochner, BS, Ritchie, ME, Hilton, DJ and Fairfax, KA ORCID: 0000-0002-8394-137X 2018 , 'Identification of a Siglec-F+ granulocyte-macrophage progenitor' , Journal of Leukocyte Biology, vol. 104, no. 1 , pp. 123-133 , doi: 10.1002/JLB.1MA1217-475R.

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Abstract

In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα- myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα- cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα- population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.

Item Type: Article
Authors/Creators:Bolden, JE and Lucas, EC and Zhou, G and O'Sullivan, JA and de Graaf, CA and McKenzie, MD and Di Rago, L and Baldwin, TM and Shortt, J and Alexander, WS and Bochner, BS and Ritchie, ME and Hilton, DJ and Fairfax, KA
Keywords: Myb, eosinophil, granulocyte, hematopoiesis, neutrophil
Journal or Publication Title: Journal of Leukocyte Biology
Publisher: Federation Amer Soc Exp Biol
ISSN: 0741-5400
DOI / ID Number: 10.1002/JLB.1MA1217-475R
Copyright Information:

Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

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