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Abstract

In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα- myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα- cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα- population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.

Item Type:	Article
Authors/Creators:	Bolden, JE and Lucas, EC and Zhou, G and O'Sullivan, JA and de Graaf, CA and McKenzie, MD and Di Rago, L and Baldwin, TM and Shortt, J and Alexander, WS and Bochner, BS and Ritchie, ME and Hilton, DJ and Fairfax, KA
Keywords:	Myb, eosinophil, granulocyte, hematopoiesis, neutrophil
Journal or Publication Title:	Journal of Leukocyte Biology
Publisher:	Federation Amer Soc Exp Biol
ISSN:	0741-5400
DOI / ID Number:	10.1002/JLB.1MA1217-475R
Copyright Information:	Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
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