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Transcriptional profiling of eosinophil subsets in interleukin-5 transgenic mice

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Fairfax, KA ORCID: 0000-0002-8394-137X, Bolden, JE, Robinson, AJ, Lucas, EC, Baldwin, TM, Ramsay, KA, Cole, R, Hilton, DJ and de Graaf, CA 2018 , 'Transcriptional profiling of eosinophil subsets in interleukin-5 transgenic mice' , Journal of Leukocyte Biology, vol. 104, no. 1 , pp. 195-204 , doi: 10.1002/JLB.6MA1117-451R.

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Abstract

Eosinophils are important in fighting parasitic infections and are implicated in the pathogenesis of asthma and allergy. IL-5 is a critical regulator of eosinophil development, controlling proliferation, differentiation, and maturation of the lineage. Mice that constitutively express IL-5 have in excess of 10-fold more eosinophils in the hematopoietic organs than their wild type (WT) counterparts. We have identified that much of this expansion is in a population of Siglec-F high eosinophils, which are rare in WT mice. In this study, we assessed transcription in myeloid progenitors, eosinophil precursors, and Siglec-F medium and Siglec-F high eosinophils from IL-5 transgenic mice and in doing so have created a useful resource for eosinophil biologists. We have then utilized these populations to construct an eosinophil trajectory based on gene expression and to identify gene sets that are associated with eosinophil lineage progression. Cell cycle genes were significantly associated with the trajectory, and we experimentally demonstrate an increasing trend toward quiescence along the trajectory. Additionally, we found gene expression changes associated with constitutive IL-5 signaling in eosinophil progenitors, many of which were not observed in eosinophils.

Item Type: Article
Authors/Creators:Fairfax, KA and Bolden, JE and Robinson, AJ and Lucas, EC and Baldwin, TM and Ramsay, KA and Cole, R and Hilton, DJ and de Graaf, CA
Keywords: Siglec-F, gene expression, granulocyte, progenitor, trajectory
Journal or Publication Title: Journal of Leukocyte Biology
Publisher: Federation Amer Soc Exp Biol
ISSN: 0741-5400
DOI / ID Number: 10.1002/JLB.6MA1117-451R
Copyright Information:

Copyright 2018 Walter and Eliza Hall Institute. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

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