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Diversity and guaiacol production of Alicyclobacillus spp. from fruit juice and fruit-based beverages

Luong, TCV, Moir, C, Kaur, M, Frank, D, Bowman, JP ORCID: 0000-0002-4528-9333 and Bradbury, MI 2019 , 'Diversity and guaiacol production of Alicyclobacillus spp. from fruit juice and fruit-based beverages' , International Journal of Food Microbiology, vol. 311 , pp. 1-6 , doi: 10.1016/j.ijfoodmicro.2019.108314.

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Abstract

Alicyclobacillus acidoterrestris is an acido-thermophilic, spore-forming bacterial species that can spoil acidic fruit juice and fruit-based beverages. The metabolism of taint compounds by this bacterial species has led to its status as a targeted microorganism in the fruit juice industry. This study aims to assess the genetic diversity of Alicyclobacillus spp. including A. acidoterrestris and its correlation to spoilage taint metabolism. Alicyclobacillus cultures, which were previously isolated from a wide range of domestic and international products including fruit juice, fruit drinks and fruit juice concentrates, were subjected to DNA fingerprint analysis by using randomly amplified polymorphic DNA (RAPD) - polymerase chain reaction. Isolates were classified on the basis of their RAPD profile and the results were used to select representative strains to undergo taint production assessment. The taint guaiacol produced by Alicyclobacillus spp. was measured by headspace gas chromatography and mass spectrometry. From produced RAPD profiles, two genotypic groups and two sub-groups were identified. The groups were independent of product types and geographical origins. A significant number of isolates were clustered in genotypic group I, including A. acidoterrestris ATCC 49025. These isolates produced significant levels of guaiacol, 8.7 mg/L on average. A smaller number of isolates was found in genotypic group II including A. acidocaldarius and they produced no guaiacol. Primer F-64 was useful to identify Alicyclobacillus at the species level, and permitted rapid identification of strains producing fruit juice taint compounds such as guaiacol.

Item Type: Article
Authors/Creators:Luong, TCV and Moir, C and Kaur, M and Frank, D and Bowman, JP and Bradbury, MI
Keywords: bacterial spores, spoilage microorganisms, acidic food stability, guaiacol RAPD-PCR, fruit juice spoilage, Alicyclobacillus
Journal or Publication Title: International Journal of Food Microbiology
Publisher: Elsevier Science Bv
ISSN: 0168-1605
DOI / ID Number: 10.1016/j.ijfoodmicro.2019.108314
Copyright Information:

Copyright 2019 Elsevier B.V.

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