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A novel and sensitive HILIC-CAD method for glucosamine quantification in plasma and its application to a human pharmacokinetic study

Asthana, C ORCID: 0000-0001-6273-2723, Peterson, GM ORCID: 0000-0002-6764-3882, Shastri, MD ORCID: 0000-0002-1012-2779 and Patel, RP ORCID: 0000-0001-9344-1013 2019 , 'A novel and sensitive HILIC-CAD method for glucosamine quantification in plasma and its application to a human pharmacokinetic study' , Journal of Pharmaceutical and Biomedical Analysis , pp. 1-9 , doi: 10.1016/j.jpba.2019.112954.

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The clinical effect of glucosamine, the most widely used supplement in patients with osteoarthritis, on joint pain andfunction improvement is reported to be inconsistent. Inter-patient variability in the pharmacokinetics of glucosamine,especially its absorption, could contribute to the inconsistent clinical outcomes. To test this hypothesis, a novel butsimple Hydrophilic Interaction Liquid Chromatography coupled with Charged Aerosol Detector method was developedand validated. The sample was prepared by simple protein precipitation and analysed using an amino column andacetonitrile:100 mM ammonium formate with gradient elution. The developed method was linear (12.5-800 ng/mL, r2 =0.999) and the relative standard deviations for intra- and inter-day accuracy, precision and repeatability were all lessthan 6%. The sensitivity of the method (lower limit of quantitation; 12.5 ng/mL) allowed the quantification ofendogenous and exogenous glucosamine levels in 12 patients with osteoarthritis, taking 1500mg glucosamine daily.The analysis showed 120-fold variation (81.7% variance) in exogenous glucosamine levels among the patients,indicating that substantial variability in the extent of absorption and/or rate of elimination could be a possible cause forthe reported inconsistent clinical outcomes. The newly-developed and sensitive method can be used to study thepharmacokinetics of glucosamine.

Item Type: Article
Authors/Creators:Asthana, C and Peterson, GM and Shastri, MD and Patel, RP
Keywords: Osteoarthritis, Glucosamine, Quantification, Plasma, HILIC-CAD, Pharmacokinetics
Journal or Publication Title: Journal of Pharmaceutical and Biomedical Analysis
Publisher: Pergamon-Elsevier Science Ltd
ISSN: 0731-7085
DOI / ID Number: 10.1016/j.jpba.2019.112954
Copyright Information:

2019 Elsevier B.V.

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