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Docosahexaenoic acid varies in rat skeletal muscle membranes according to fibre type and provision of dietary fish oil

Macartney, MJ ORCID: 0000-0001-7265-5725, Peoples, GE, Treweek, TM and McLennan, PL 2019 , 'Docosahexaenoic acid varies in rat skeletal muscle membranes according to fibre type and provision of dietary fish oil' , Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 151 , pp. 37-44 , doi: 10.1016/j.plefa.2019.08.006.

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Abstract

Background: Dietary fish oil provides polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA) and isassociated with modified oxygen consumption, contractile fatigue and physiological responses to ischaemia orhypoxia in striated muscle. This study systematically investigated the membrane incorporation of fatty acids,with a focus on DHA, into skeletal muscle in relation to functional/metabolic differences and their responsiveness to fish oil doses.Methods: Male Sprague-Dawley rats were randomised to isoenergetic diets (10% fat by weight). Human Westernstyle diets were simulated with 5.5% tallow, 2.5% n-6 PUFA sunflower seed oil and 2% olive oil (Control). HighDHA tuna oil exchanged for olive oil provided a Low (0.32%) or moderate (Mod) (1.25%) fish oil diet.Membrane phospholipid fatty acid composition was analysed in samples of five skeletal muscles selected formaximum variation in muscle fibre-type.Results: Concentrations of DHA varied according to muscle fibre type, very strongly associated with fast oxidative glycolytic fibre population (r2 = 0.93; P Conclusion: The exquisite responsiveness of all skeletal muscles to as little fish oil as the equivalent of 1–2 fishmeals per week in a human diet and the selective relationship to fatigable muscle fibre-types supports an integralrole for DHA in muscle physiology, and particularly in fatigue resistance of fast-twitch muscles.Summary: Skeletal muscle fibres vary according to structural, metabolic and neurological characteristics andultimately influences contractile function. This study sort to determine if the composition of phospholipidpolyunsaturated fatty acids (PUFA), incorporated in their membranes, might also differ according to fibre typeand when omega-3 PUFA are made available in the diet. We systematically demonstrated that the omega-3PUFA, docosahexaenoic acid (DHA), incorporated into skeletal muscle membranes well above its provision in thediet and without competitive influence of high omega-6 PUFA concentrations, typical to the Western-stylehuman diet. Notably, incorporation preferentially occurred according to metabolic characteristics of eachmuscle, supporting the notion that DHA plays an integral role in fast oxidative glycolytic muscle fibres.

Item Type: Article
Authors/Creators:Macartney, MJ and Peoples, GE and Treweek, TM and McLennan, PL
Keywords: polyunsaturated fatty acids, Omega-3, contractile fatigue, fast oxidative glycolytic, fast-twitch muscle
Journal or Publication Title: Prostaglandins Leukotrienes and Essential Fatty Acids
Publisher: Churchill Livingstone
ISSN: 0952-3278
DOI / ID Number: 10.1016/j.plefa.2019.08.006
Copyright Information:

Copyright 2019 Elsevier Ltd.

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