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The effect of sleep restriction, with or without high-intensity interval exercise, on myofibrillar protein synthesis in healthy young men


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Saner, NJ, Lee, MJ, Pitchford, NW ORCID: 0000-0003-3169-9347, Kuang, J, Roach, GD, Garnham, A, Stokes, T, Phillips, SM, Bishop, DJ and Bartlett, JD 2020 , 'The effect of sleep restriction, with or without high-intensity interval exercise, on myofibrillar protein synthesis in healthy young men' , Journal of Physiology , pp. 1-14 , doi: 10.1113/JP278828.

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The present study aimed to investigate the effect of sleep restriction, with orwithout high-intensity interval exercise (HIIE), on the potential mechanisms underpinningpreviously-reported sleep-loss-induced reductions to muscle mass. Twenty-four healthy, youngmen underwent a protocol consisting of two nights of controlled baseline sleep and a five-nightintervention period. Participants were allocated into one of three parallel groups, matched for age,V˙O2peak , body mass index and habitual sleep duration; a normal sleep (NS) group [8 h time in bed(TIB) each night], a sleep restriction (SR) group (4 h TIB each night), and a sleep restriction and exercise group (SR+EX, 4 h TIB each night, with three sessions of HIIE). Deuterium oxide wasingested prior to commencing the study and muscle biopsies obtained pre- and post-interventionwere used to assess myofibrillar protein synthesis (MyoPS) and molecular markers of proteinsynthesis and degradation signalling pathways. MyoPS was lower in the SR group [fractionalsynthetic rate (% day–1), mean ± SD, 1.24 ± 0.21] compared to both the NS (1.53 ± 0.09)and SR+EX groups (1.61 ± 0.14) (P ser473 and p-mTORser2448) and degradation (i.e. Foxo1/3mRNA and LC3 protein) in any group. These data suggest that MyoPS is acutely reduced bysleep restriction, although MyoPS can be maintained by performing HIIE. These findings mayexplain the sleep-loss-induced reductions in muscle mass previously reported and also highlightthe potential therapeutic benefit of HIIE to maintain myofibrillar remodelling in this context.

Item Type: Article
Authors/Creators:Saner, NJ and Lee, MJ and Pitchford, NW and Kuang, J and Roach, GD and Garnham, A and Stokes, T and Phillips, SM and Bishop, DJ and Bartlett, JD
Keywords: Sleep restriction, diabetes, insulin, high-intensity exercise
Journal or Publication Title: Journal of Physiology
Publisher: Cambridge Univ Press
ISSN: 0022-3751
DOI / ID Number: 10.1113/JP278828
Copyright Information:

Copyright 2020 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. DOI: 10.1113/JP278828This is an open access article under the terms of the Creative Commons Attribution License (CC-BY), which permits use, distributionand reproduction in any medium, provided the original work is properly cited.

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