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Cloned ermTR gene confers low level erythromycin but high level clindamycin resistance in Streptococcus pyogenes NZ131

Oryasin, E, Biyik, HH, Tristram, S ORCID: 0000-0002-8485-0322 and Bozdogan, B 2020 , 'Cloned ermTR gene confers low level erythromycin but high level clindamycin resistance in Streptococcus pyogenes NZ131' , Microbial Drug Resistance , doi: 10.1089/mdr.2019.0133.

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Objectives: The most common macrolide resistance mechanisms in streptococci are the presence of methylase encoding genes ermB and ermTR or the presence of efflux encoded by mef genes. In the present study we aimed to show the effects of the ermTR gene under isogenic conditions on the activities of macrolides and lincosamides in streptococci.Materials and Methods: Total DNA was extracted from Streptococcus pyogenes C1, and the ermTR gene was amplified with or without the regulatory region using modified primer with insertion of restriction sites to clone in to pUC18. Transformants were selected after electroporation of Escherichia coli DB10. The recombinant plasmids were purified and merged to pJIM2246 to transform Gram positive bacteria. Recombinant pJIM2246 plasmids with the ermTR gene were then introduced into S. pyogenes NZ131 by electroporation.Results: After transformation with ermTR without regulatory region the minimal inhibitory concentration (MIC) for erythromycin and clindamycin increased from ≤0.06 to ≤0.06 to 8 and >128 mg/L, respectively. Induction with erythromycin affected the MICs for clindamycin of S. pyogenes transformed with ermTR with the regulatory region. Double disk testing showed that induction with erythromycin and azithromycin for the S. pyogenes transformed with ermTR, and regulatory regions decreased the clindamycin inhibition zone but not telithromycin. The ermTR gene in isogenic conditions confers low level resistance to erythromycin and high level resistance to clindamycin.Conclusion: The different induction and resistance profiles of ermTR compared to other erm genes suggest that the methylation of ErmTR may be different than well studied methylases.

Item Type: Article
Authors/Creators:Oryasin, E and Biyik, HH and Tristram, S and Bozdogan, B
Keywords: antibiotic, Haemophilus macrolide
Journal or Publication Title: Microbial Drug Resistance
Publisher: Mary Ann Liebert Inc Publ
ISSN: 1076-6294
DOI / ID Number: 10.1089/mdr.2019.0133
Copyright Information:

Copyright 2020 Mary Ann Liebert, Inc.

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