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Change of Ras and its guanosine triphosphatases (GTPases) during development and regression in bovine corpus luteum

Lee, SH and Lee, S 2020 , 'Change of Ras and its guanosine triphosphatases (GTPases) during development and regression in bovine corpus luteum' , Theriogenology, vol. 144 , pp. 16-26 , doi: 10.1016/j.theriogenology.2019.12.014.

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The aim of this study was to determine the change of Ras and its guanosine triphosphatases (GTPases) proteins in the bovine corpus luteum (CL) during estrous cycle and investigate protein-protein interaction between hormone receptors and Ras proteins via angiogenetic and apoptotic factors using bioinformatics database. The bovine CLs at proliferation phase (PP), secretion phase (SP), and regression phase (RP) were dissected from abattoir ovaries (n = 4/stage), whole of the tissue samples was used to analyze two-dimensional electrophoresis (2-DE), mRNA, and protein analysis. The protein-protein interaction between the Ras GTPases proteins and hormone receptors were analyzed using Search Tool for the Retrieval of Interacting Genes (STRING) database. The Ras protein activator like 3 (RASAL3), Ras GTPase activating protein 3 (RASA3), Ras guanine nucleotide exchange factors 1 beta (RasGEF1B) were discovered by the 2-DE and mass spectrometry in bovine CLs, and the protein spots of RASA3 and RASAL3 were significantly increased in the SPCL compared to the PPCL, whereas the RasGEF1B was reduced in the PPCL (P P P < 0.05). In summary, the RasGEF and Ras proteins were raised during the development, whereas the RasGAP was increased when development was completed, then the Ras and its GTPases dramatically decreased at the regression in bovine CL. In conclusion, these results suggest that Ras and Ras GTPases could be changed during development and regression, activated by the ERα via angiogenetic signaling during proliferation, and may be important to understanding of the Ras and its GTPases system for estrous cycle in bovine CL.

Item Type: Article
Authors/Creators:Lee, SH and Lee, S
Keywords: Ras, Ras GTPases, corpus luteum, cows
Journal or Publication Title: Theriogenology
Publisher: Elsevier Science Inc
ISSN: 0093-691X
DOI / ID Number: 10.1016/j.theriogenology.2019.12.014
Copyright Information:

© 2019 Elsevier Inc. All rights reserved.

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