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Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation

Dorantes-Aranda, JJ ORCID: 0000-0003-1513-6501, Hayashi, A ORCID: 0000-0002-7348-9682, Turnbull, AR ORCID: 0000-0001-5701-8728, Jolley, JYC, Harwood, DT and Hallegraeff, GM ORCID: 0000-0001-8464-7343 2020 , 'Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation' , Journal of AOAC International, vol. 103, no. 3 , pp. 784-791 , doi: 10.1093/jaocint/qsz029.

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Abstract

BackgroundParalytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money.ObjectiveThe aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster.MethodsThe AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4).ResultsThe Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences.ConclusionsThe results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster.HighlightsThe Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas.

Item Type: Article
Authors/Creators:Dorantes-Aranda, JJ and Hayashi, A and Turnbull, AR and Jolley, JYC and Harwood, DT and Hallegraeff, GM
Keywords: PST test kit validation, rock lobster
Journal or Publication Title: Journal of AOAC International
Publisher: Aoac International
ISSN: 1060-3271
DOI / ID Number: 10.1093/jaocint/qsz029
Copyright Information:

Copyright 2020 AOAC International

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