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Cow dung biomass smoke exposure increases adherence of respiratory pathogen nontypeable Haemophilus infuenzae to human bronchial epithelial cells

KC, R, Hyland, IKP ORCID: 0000-0001-9850-0238, Smith, JA ORCID: 0000-0001-6313-3298, Shukla, SD, Hansbro, PM, Zosky, GR ORCID: 0000-0001-9039-0302, Karupiah, G ORCID: 0000-0001-6306-4405 and O'Toole, RF ORCID: 0000-0002-4579-4479 2020 , 'Cow dung biomass smoke exposure increases adherence of respiratory pathogen nontypeable Haemophilus infuenzae to human bronchial epithelial cells' , Exposure and Health , doi: 10.1007/s12403-020-00351-y.

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Abstract

Biomass smoke exposure is associated with a heightened risk of development of respiratory diseases that include chronicobstructive pulmonary disease (COPD). The aim of this study was to increase our understanding of how biomass smokecould contribute to an increased susceptibility to respiratory infection. We investigated the efects of cow dung and woodsmoke exposure on human bronchial epithelial cells with respect to adherence of a major respiratory bacterial pathogen inCOPD, nontypeable Haemophilus infuenzae (NTHi), using immunofuorescence microscopy. In addition, expression of aknown receptor of NTHi, platelet-activating factor receptor (PAFR), and two pro-infammatory cytokines, interleukin 6 (IL6) and interleukin-8 (IL-8), were determined using quantitative polymerase chain reaction. We observed a dose-dependentincrease in NTHi adhesion to human bronchial epithelial cells following exposure to cow dung but not wood smoke extracts.Pre-treatment with PAFR antagonists, WEB-2086 and its analogue, C17, decreased adherence by NTHi to airway epithelialcells exposed to cow dung smoke. Both cow dung and wood smoke-induced expression of PAFR, as well as of IL-6 andIL-8, which was inhibited by WEB-2086 and C17. In conclusion, biomass smoke from combustion of cow dung and woodinduced expression of PAFR and airway infammatory markers in human bronchial epithelial cells. Cow dung exposure, butnot wood smoke exposure, mediated a measurable increase in NTHi adhesion to airway epithelial cells that was inhibited byPAFR antagonists. This work highlights the potential of PAFR as a therapeutic target for reducing the impact of hazardousbiomass smoke exposure on respiratory health.

Item Type: Article
Authors/Creators:KC, R and Hyland, IKP and Smith, JA and Shukla, SD and Hansbro, PM and Zosky, GR and Karupiah, G and O'Toole, RF
Keywords: chronic obstructive pulmonary disease, cow dung smoke, biomass smoke, cigarette smoke, nontypeable, <i>Haemophilus infuenzae</i>
Journal or Publication Title: Exposure and Health
Publisher: Springer Netherlands
ISSN: 2451-9766
DOI / ID Number: 10.1007/s12403-020-00351-y
Copyright Information:

© Springer Nature B.V. 2020

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