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Influences of detector time constant variations on efficiency calculations in the standardisation of high performance liquid chromatographic columns

journal contribution
posted on 2023-05-26, 10:57 authored by Low, GKC, Paul HaddadPaul Haddad
This paper reports the influences of detector time constant variations on plate count calculations and peak retention times in the standardisation of high-performance liquid chromatographic columns. A recommendation is made here that column efficiencies should be quoted at zero time constant in order to remove the variability in plate count introduced by varying time constant. For detectors which do not have variable time constant controls, it is recommended that the calculation of plate numbers should be made on solutes having capacity factors in the region 5‚Äö-6. Further recommendations made are that the peak symmetry correlation ratio should be used to evaluate system performance only when calculated at zero time constant and that the detector time constant should not exceed one hundredth of the peak width for peaks used in the calculation of plate numbers.

History

Publication title

Journal of Chromatography A

Volume

198

Article number

3

Number

3

Pagination

235-239

ISSN

0021-9673

Publication status

  • Published

Rights statement

The definitive version is available at http://www.sciencedirect.com

Repository Status

  • Restricted

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