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Isolation and complete genome sequence of a novel cyanophage, S‑B05, infecting an estuarine Synechococcus strain: insights into environmental adaptation

Jiang, Tong, Guo, C, Wang, M, Wang, M, You, S, Liu, Y, Zhang, X, Liu, H, Jiang, Y, Shao, H, Liang, Y and McMinn, A ORCID: 0000-0002-2133-3854 2020 , 'Isolation and complete genome sequence of a novel cyanophage, S‑B05, infecting an estuarine Synechococcus strain: insights into environmental adaptation' , Archives of Virology, vol. 165 , 1397–1407 , doi: 10.1007/s00705-020-04595-6.

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Abstract

A new cyanophage, S-B05, infecting a phycoerythrin-enriched (PE-type) Synechococcus strain was isolated by the liquid infection method, and its morphology and genetic features were examined. Phylogenetic analysis and morphological observation confirmed that S-B05 belongs to the family Myoviridae of the order Caudovirales. Its genome was fully sequenced, and found to be 208,857 bp in length with a G + C content of 39.9%. It contained 280 potential open reading frames and 123 conserved domains. Ninety-eight functional genes responsible for cyanophage structuring and packaging, DNA replication and regulation, and photosynthesis were identified, as well as genes encoding 172 hypothetical proteins. The genome of S-B05 is most similar to that of Prochlorococcus phage P-TIM68. Homologues of open reading frames of S-B05 can be found in various marine environments, as revealed by comparison of the S-B05 genome sequence to sequences in marine viral metagenomic databases. The presence of auxiliary metabolic genes (AMGs) related to photosynthesis, carbon metabolism, and phosphorus assimilation, as well as the phylogenetic relationships based on AMGs and the complete genome sequence, reflect the phage-host interaction mechanism or the specific adaptation strategy of the host to environmental conditions. The genome sequence information reported here will provide an important basis for further study of the adaptive evolution and ecological role of cyanophages and their hosts in the marine environment.

Item Type: Article
Authors/Creators:Jiang, Tong and Guo, C and Wang, M and Wang, M and You, S and Liu, Y and Zhang, X and Liu, H and Jiang, Y and Shao, H and Liang, Y and McMinn, A
Keywords: cyanobacteria, genome, Synechococcus
Journal or Publication Title: Archives of Virology
Publisher: Springer-Verlag Wien
ISSN: 0304-8608
DOI / ID Number: 10.1007/s00705-020-04595-6
Copyright Information:

© Springer-Verlag GmbH Austria, part of Springer Nature 2020

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