PDF 016.pdf | Request a copy Full text restricted Available under University of Tasmania Standard License.

Abstract

In the determination of ephedrine using reversed-phase, ion-pair liquid chromatography, a chromatographically pure sample was observed to give three peaks under certain mobile phase conditions. The mobile phases which produced maximum peak splitting were determined for ephedrine and a number of other sympathomimetic drugs.
A proposal that peak splitting was the result of the composite interplay of two discrete chromatographic mechanisms, was investigated. The results of analysis by GC/MS confirmed that each peak was due to ephedrine, however, only one of the three split peaks was found to contain ion pairs. It is postulated that peak splitting is a physical phenomenon on reversed-phase columns and the separation of these drugs by ion-pair HPLC is based on a mixed rather than a single mechanism.
This study has also shown that errors can arise in ion-pair HPLC when multiple peaks are assumed to indicate heterogeneity. Interconvertible species of the same solute can give rise to these peaks.

Item Type:	Article
Authors/Creators:	Low, GKC and Duffield, AM and Haddad, PR
Keywords:	Ion-pair liquid chromatography - Peak splitting - Sympathominetic drugs
Journal or Publication Title:	Chromatographia
ISSN:	0009-5893 (Print) 1612-1112 (Online)
DOI / ID Number:	https://doi.org/10.1007/BF02265679
Additional Information:	The original publication is available at http://www.springerlink.com/
Item Statistics:	View statistics for this item

Actions (login required)

Item Control Page