Open Access Repository

Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism

Low, GKC, Duffield, AM and Haddad, PR 1982 , 'Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism' , Chromatographia, vol. 15, no. 5 , pp. 289-296 , doi:

[img] PDF
016.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.


In the determination of ephedrine using reversed-phase, ion-pair liquid chromatography, a chromatographically pure sample was observed to give three peaks under certain mobile phase conditions. The mobile phases which produced maximum peak splitting were determined for ephedrine and a number of other sympathomimetic drugs.
A proposal that peak splitting was the result of the composite interplay of two discrete chromatographic mechanisms, was investigated. The results of analysis by GC/MS confirmed that each peak was due to ephedrine, however, only one of the three split peaks was found to contain ion pairs. It is postulated that peak splitting is a physical phenomenon on reversed-phase columns and the separation of these drugs by ion-pair HPLC is based on a mixed rather than a single mechanism.
This study has also shown that errors can arise in ion-pair HPLC when multiple peaks are assumed to indicate heterogeneity. Interconvertible species of the same solute can give rise to these peaks.

Item Type: Article
Authors/Creators:Low, GKC and Duffield, AM and Haddad, PR
Keywords: Ion-pair liquid chromatography - Peak splitting - Sympathominetic drugs
Journal or Publication Title: Chromatographia
ISSN: 0009-5893 (Print) 1612-1112 (Online)
DOI / ID Number:
Additional Information:

The original publication is available at

Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page