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Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism


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Low, GKC and Duffield, AM and Haddad, PR (1982) Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism. Chromatographia, 15 (5). pp. 289-296. ISSN 0009-5893 (Print) 1612-1112 (Online)

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In the determination of ephedrine using reversed-phase, ion-pair liquid chromatography, a chromatographically pure sample was observed to give three peaks under certain mobile phase conditions. The mobile phases which produced maximum peak splitting were determined for ephedrine and a number of other sympathomimetic drugs.
A proposal that peak splitting was the result of the composite interplay of two discrete chromatographic mechanisms, was investigated. The results of analysis by GC/MS confirmed that each peak was due to ephedrine, however, only one of the three split peaks was found to contain ion pairs. It is postulated that peak splitting is a physical phenomenon on reversed-phase columns and the separation of these drugs by ion-pair HPLC is based on a mixed rather than a single mechanism.
This study has also shown that errors can arise in ion-pair HPLC when multiple peaks are assumed to indicate heterogeneity. Interconvertible species of the same solute can give rise to these peaks.

Item Type: Article
Keywords: Ion-pair liquid chromatography - Peak splitting - Sympathominetic drugs
Journal or Publication Title: Chromatographia
Page Range: pp. 289-296
ISSN: 0009-5893 (Print) 1612-1112 (Online)
Identification Number - DOI: 10.1007/BF02265679
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Date Deposited: 24 Mar 2008 10:16
Last Modified: 18 Nov 2014 03:31
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