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Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry



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Abstract
The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct “on-thread” ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.
Item Type: | Article |
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Authors/Creators: | Chen, L and Cabot, JM and Sanz Rodriguez, E and Ghiasvand, A and Innis, PC and Paull, B |
Keywords: | thread-based isoelectric focusing, desorption electrospray ionization mass spectrometry |
Journal or Publication Title: | Analyst |
Publisher: | Royal Soc Chemistry |
ISSN: | 0003-2654 |
DOI / ID Number: | 10.1039/d0an01344g |
Copyright Information: | © The Royal Society of Chemistry 2020 |
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Item Statistics: | View statistics for this item |
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