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Abstract

The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct “on-thread” ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.

Item Type:	Article
Authors/Creators:	Chen, L and Cabot, JM and Sanz Rodriguez, E and Ghiasvand, A and Innis, PC and Paull, B
Keywords:	thread-based isoelectric focusing, desorption electrospray ionization mass spectrometry
Journal or Publication Title:	Analyst
Publisher:	Royal Soc Chemistry
ISSN:	0003-2654
DOI / ID Number:	10.1039/d0an01344g
Copyright Information:	© The Royal Society of Chemistry 2020
Related URLs:	UTAS Profile: Cabot, JM UTAS Profile: Sanz Rodriguez, E Google Scholar: Ghiasvand, A Google Scholar: Paull, B UTAS Profile: Paull, B
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