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High Performance Liquid Chromoatography of some analgesic compounds: an instrumental analysis experiment.


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Haddad, PR, Hutchins, SR and Tuffy, MB 1983 , 'High Performance Liquid Chromoatography of some analgesic compounds: an instrumental analysis experiment.' , Journal of Chemical Education, vol. 60, no. 2 , pp. 166-168 .

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The technique of High Performance Liquid Chromatography
(LC) is a useful method for a wide variety of analyses
and may be used to complement the older technique of gas
chromatography. Much of the work currently puhlished on
LC is, however, not directly suitable to illustrate the wide
variety of procedures which may he used to enhance the separation characteristics of a mixture, while at the same time, prove interesting and relevant as an undergraduate experiment.
Previously published student experiments on LC include
analysis of essential oils ( I ) , analysis of urinary compounds
(21, and liquid chromatography with electrochemical
detection (3). These experiments provide interesting examples
of the application of LC but are not designed to illustrate the
effects of such fundamental variables as solvent composition,
pH, etc. In this paper we present an experiment which demonstrates
the techniques of solvent selection, gradient elution,
pH control, and ion-pairing in the analysis of an analgesic
mixture using reversed phase LC on an octadecylsilane (ODs)
column. Although this experiment has been developed using
fairly sophisticated apparatus, the majority of the procedures
described are directly applicable to inexpensive, isocratic
instruments. Selection of a suitahle erouu of solutes for the experiment was based in part on a published evaluation of the applicability of reversed phase LC to drug analysis (4); the selected compounds are shown in Figure 1.

Item Type: Article
Authors/Creators:Haddad, PR and Hutchins, SR and Tuffy, MB
Journal or Publication Title: Journal of Chemical Education
ISSN: 0021-9584
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